Products:Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cytochromes
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Has this antibody been tested on other cytochrome P450 enzymes other than IIE1? |
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ANSWER: |
Thank you for your enquiry. The antibody has not been specifically tested with other cytochrome P450 enzymes. It is unknown which enzymes, if any, this would cross-react with other than IIE1. I am sorry I could not be more helpful. Please do not hesitate to contact us if you need anything further. |
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What type of product can this be? There are at least 15 different P450 enzymes in rat liver. An antibody made to one enzyme can cross-react with several others. The description of this product leaves much to be desired.
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ANSWER: |
This antibody was raised to cytochrome P450 IIE1 purified from rat liver. This enzyme is also referred to as CYP2E1. I am sorry that this information was not included in our data sheet for this product, but I have subsequently added it. I hope this helps. Please let me know if you need anything else. Thank you! |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
ab19140 staining Cytochrome P450 2E1 in Human 293FT cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilzed with 0.1% Trton X-100 and blocked with 5% serum for 1 hour at 25ºC. Samples were incubated with primary antibody (1/200 in PBS) for 2 hours at 25ºC. An Alexa Fluor®488-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
ab19140 staining Cytochrome P450 2E1 in rat bone marrow cells by Immunocytochemistry/ Immunofluorescence.The cells were formaldehyde fixed, permeabilised in 0.1% Triton X-100 and then blocked using 5% serum for 1 hour at 25ºC. Samples were then incubated with primary antibody at 1/200 for 12 hours at 4ºC. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/250 dilution. DAPI was used to stain the cell nuclei (blue).
Image courtesy of an anonymous Abreview
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