Anti-Cytochrome P450 2E1 antibody (ab19140)

  Immunocytochemistry/ Immunofluorescence - Cytochrome P450 2E1 antibody (ab19140)

ab19140 staining Cytochrome P450 2E1 in Human 293FT cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilzed with 0.1% Trton X-100 and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/200 in PBS) for 2 hours at 25°C. An Alexa Fluor^®488-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

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  Immunocytochemistry/ Immunofluorescence - Cytochrome P450 2E1 antibody (ab19140)

ab19140 staining Cytochrome P450 2E1 in rat bone marrow cells by Immunocytochemistry/ Immunofluorescence.

The cells were formaldehyde fixed, permeabilised in 0.1% Triton X-100 and then blocked using 5% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/200 for 12 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/250 dilution. DAPI was used to stain the cell nuclei (blue).

Image courtesy of an anonymous Abreview

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