Products:Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cytochromes
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab28146 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
|
|||||||||
|
|||||||||
Dear Abcam representative, |
|||||||||
ANSWER: |
Thank you for contacting us. |
||||||||
|
|||||||||
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
ab28146 staining cells of rat liver sections by IHC-Fr. Cells were fixed in acetone and blocked with 1% BSA for 5 minutes at 25°C prior to incubating with ab28146 diluted 1/300 for 30 minutes at 25°C. An Alexa Fluor® 594 conjugated goat anti-rabbit antibody was used as the secondary.
Double IF labeling with CYP2E1 (Red) and dipeptidyl peptidase IV (Green)
This image is courtesy of an anonymous Abreview
ICC/IF image of ab28146 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28146, 1/1000 dilution) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/1000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Cell lysates prepared from human liver microsomes
Lane 3 : Cell lysates prepared from rat liver microsomes
Lane 4 : Cell lysates prepared from mouse liver microsomes
Lane 5 : Cell lysates prepared from rabbit liver microsomes
Predicted band size : 50-55 kDa
All lanes : Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/2500 dilution
Lane 1 : Tissue lysate prepared from normal murine liver
Lane 2 : Tissue lysate prepared from normal murine liver
Lane 3 : Tissue lysate prepared from normal murine liver
Lane 4 : Tissue lysate prepared from normal murine liver
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-rabbit IgG-HRP at 1/5000 dilution
developed using the ECL technique
Predicted band size : 50-55 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 seconds
Image courtesy of an anonymous Abreview.
3
Call 01223 696 000 or contact us