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Products:Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cytochromes
Overview
Product name
Anti-Cytochrome P450 2E1 antibody
See all Cytochrome P450 2E1 products (11) ...
Description
Rabbit polyclonal to Cytochrome P450 2E1
Specificity
The antibody detects a ~50-55 kDa protein, corresponding to the apparent molecular mass of cytochrome P450 IIE1 on SDS-PAGE immunoblots.
Tested applications
IHC-Fr, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Human, Monkey
Immunogen
Full length native protein (purified) (Rat)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.2
Purity
Whole antiserum
Primary antibody notes
The antibody has been shown to inhibit microsomal benzene metabolism
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Alcohol metabolism
Signal Transduction >> Metabolism >> Drug metabolism
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cytochromes
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: 1/300(PubMed: 17021035)
WB: 1/5000Predicted molecular weight: 50-55 kDa.(This dilution was sufficient for detection of Cytochrome P450 in 20ug of human liver microsomes by colorimetric immunoblot analysis using Goat anti-Rabbit IgG:AP as the secondary antibody. )
ICC/IF: 1/1000
Target
Function
Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms.
Sequence similarities
Belongs to the cytochrome P450 family.
Cellular localization
Endoplasmic reticulum membrane. Microsome membrane.
Target information above from: UniProt accessionP05181
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 4-nitrophenol 2-hydroxylase antibody
- CP2E1_HUMAN antibody
- CPE 1 antibody
see all
Database links
- Entrez Gene: 1571 Human
- Entrez Gene: 13106 Mouse
- Entrez Gene: 25086 Rat
- Omim: 124040 Human
- SwissProt: P05181 Human
- SwissProt: Q05421 Mouse
- SwissProt: P08682 Rabbit
- SwissProt: P05182 Rat
see all
Anti-Cytochrome P450 2E1 antibody images:
Immunohistochemistry (Frozen sections) - Cytochrome P450 2E1 antibody (ab28146)
ab28146 staining cells of rat liver sections by IHC-Fr. Cells were fixed in acetone and blocked with 1% BSA for 5 minutes at 25°C prior to incubating with ab28146 diluted 1/300 for 30 minutes at 25°C. An Alexa Fluor® 594 conjugated goat anti-rabbit antibody was used as the secondary.
Double IF labeling with CYP2E1 (Red) and dipeptidyl peptidase IV (Green)
This image is courtesy of an anonymous Abreview
Immunocytochemistry/ Immunofluorescence - Cytochrome P450 2E1 antibody (ab28146)
ICC/IF image of ab28146 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28146, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Cytochrome P450 2E1 antibody (ab28146)
All lanes : Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/1000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Cell lysates prepared from human liver microsomes
Lane 3 : Cell lysates prepared from rat liver microsomes
Lane 4 : Cell lysates prepared from mouse liver microsomes
Lane 5 : Cell lysates prepared from rabbit liver microsomes
Predicted band size : 50-55 kDa
Western blot - Cytochrome P450 2E1 antibody (ab28146)
All lanes : Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/2500 dilution
Lane 1 : Tissue lysate prepared from normal murine liver
Lane 2 : Tissue lysate prepared from normal murine liver
Lane 3 : Tissue lysate prepared from normal murine liver
Lane 4 : Tissue lysate prepared from normal murine liver
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-rabbit IgG-HRP at 1/5000 dilution
developed using the ECL technique
Predicted band size : 50-55 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 seconds
Image courtesy of an anonymous Abreview.
References for Anti-Cytochrome P450 2E1 antibody (ab28146)
This product has been referenced in:
- Piguet ACet al. Hypoxia aggravates non-alcoholic steatohepatitis in mice lacking hepatocellular PTEN. Clin Sci (Lond) 118:401-10 (2010). WB; Mouse.Read more (PubMed: 19832698) »
- Posadas Iet al. Acetaminophen induces apoptosis in rat cortical neurons. PLoS One 5:e15360 (2010). WB; Rat.Read more (PubMed: 21170329) »
See all 9 publications for this product
Publishing research using ab28146? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"