Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-Cytochrome P450 3A4 antibody
See all Cytochrome P450 3A4 products (5) ...
Description
Rabbit polyclonal to Cytochrome P450 3A4
Specificity
Detects Cytochrome P450 3A4 from human and rat tissues and cells. This antibody detects, to a lesser extent, other P450 isoforms ( may detect the Cytochrome P450 1A2, but this band should not be as strong).
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
Purified His-tagged full length human P450 3A4 fusion protein.
Positive control
Rat liver lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: PBS, 1mg/ml BSA
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Cytochromes
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Drug metabolism
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Lipases
Signal Transduction >> Metabolism >> Drug metabolism
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cytochromes
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000. Detects a band of approximately 52 kDa (predicted molecular weight: 57 kDa).A 46kDa band is detected in rat liver extracts.
IHC-P: Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Target
Function
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide.
Tissue specificity
Expressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056). According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703). Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level).
Sequence similarities
Belongs to the cytochrome P450 family.
Post-translational
modifications
Polyubiquitinated in the presence of AMFR and UBE2G1 and also STUB1/CHIP and UBE2D1 (in vitro).
Cellular localization
Endoplasmic reticulum membrane. Microsome membrane.
Target information above from: UniProt accessionP08684
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Albendazole monooxygenase antibody
- Albendazole sulfoxidase antibody
- CP33 antibody
see all
Database links
- Entrez Gene: 1576 Human
- Entrez Gene: 286929 Rat
- Omim: 124010 Human
- SwissProt: P08684 Human
- SwissProt: P04800 Rat
- Unigene: 442527 Human
- Unigene: 728751 Human
Anti-Cytochrome P450 3A4 antibody images:
- Cytochrome P450 3A4 antibody (ab3572)
ab3572 at a dilution of 1/2000 staining cytochrome P450 3A4 in rat liver extract by Western blot.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytochrome P450 3A4 antibody (ab3572)
ab3572 (1µg/ml) staining cytochrome P450 3A4 in human Liver using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasm of the plates of hepatic cells .
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Western blot - Cytochrome P450 3A4 antibody (ab3572)
Anti-Cytochrome P450 3A4 antibody (ab3572) at 1/500 dilution +
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 57 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
Additional bands at : 26 kDa,29 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 90 seconds
References for Anti-Cytochrome P450 3A4 antibody (ab3572)
This product has been referenced in:
- Li Y et al. Induction of CYP3A4 and MDR1 gene expression by baicalin, baicalein, chlorogenic acid, and ginsenoside Rf through constitutive androstane receptor- and pregnane X receptor-mediated pathways. Eur J Pharmacol 640:46-54 (2010). WB; Human.Read more (PubMed: 20580705) »
- Irusta G et al. Dynamic expression of epoxyeicosatrienoic acid synthesizing and metabolizing enzymes in the primate corpus luteum. Mol Hum Reprod 13:541-8 (2007).Read more (PubMed: 17567599) »
See all 2 publications for this product
Publishing research using ab3572? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"