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Cytochrome c Human ELISA Kit (with color giving dyes) (ab119521)

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Overview

  • Product nameCytochrome c Human ELISA Kit (with color giving dyes)See all Cytochrome c kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 6%
  • Tests
    1 x 96 test
  • Sample type
    Cell Lysate
  • Assay typeSandwich
  • Sensitivity
    0.05 ng/ml
  • Range
    0.08 ng/ml - 5 ng/ml
  • Recovery

    82 %

    Sample specific recovery
    Sample type Average % Range
    Cell Lysate 98 78% - 119%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab119521 Cytochrome c Human ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of human Cytochrome c. ab119521 is for research use only. Not for diagnostic or therapeutic procedures.

    Cytochrome c was identified as a component required for the crucial steps in apoptosis, caspase-3 activation and DNA fragmentation. Cytochrome c was shown to redistribute from mitochondria to cytosol during apoptosis in intact cells.

    Mitochrondrial cytochrome c is a water-soluble protein of 15 kDa with a net positive charge, residing loosely attached in the mitochrondrial intermembrane space. Cytochrome c functions in the respiratory chain by interaction with redox partners. The release of cytochrome c into the cytosol leads to an activation of an apoptotic program via activation of a caspase dependent pathway. Cytochrome c achieves this goal by interaction with other cytosolic factors forming a complex (apoptosome) composed of cytochrome c, Apaf-1, dATP and Apaf-3/caspase 9. Bcl-2 on the other hand was shown to be able to prevent apoptosis by blocking the release of cytochrome c from mitochondria. Measurement of cytochrome c release from the mitochondria is a tool to detect the first early steps for initiating apoptosis in cells. Moreover, elevated cytochrome c levels were observed in serum from patients with hematological malignancies. In the course of cancer chemotherapy, the serum-cytochrome c level grew rapidly and it decreased gradually as the patient was cleared from malignant cells. Thus, serum-cytochrome c monitoring might serve as a clinical marker indicating the onset of apoptosis and cell turn-over in vivo.

    In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.

  • Tested applicationsSandwich ELISA more details

Properties

  • RelevanceElectron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
  • Cellular localizationMitochondrion matrix
    • Alternative names
        CYCCYCSCytochrome c somatic
        HCSTHC4
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab119521 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Cytochrome c Human ELISA Kit (with color giving dyes) images

      • Sandwich ELISA - Cytochrome c Human ELISA Kit (with color giving dyes) (ab119521)
        Sandwich ELISA - Cytochrome c Human ELISA Kit (with color giving dyes) (ab119521)
        Representative standard curve using ab119521.

      Protocols

      References for Cytochrome c Human ELISA Kit (with color giving dyes) (ab119521)

      ab119521 has not yet been referenced specifically in any publications.

      Publishing research using ab119521 ? Please let us know so that we can cite the reference in this datasheet

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      I lysed my cells few weeks ago using the lysis buffer that was supplied with [ab119507 Caspase-8 Human ELISA Kit]. I would like to run another ELISA experiment to detect cytochrome C release from the mitochondria, can I use the cell lysate that I have fr...

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      The lysis buffer in kits ab119507 and ab119521 are identical, so you can analyze lysates prepared for ab119507 with ab119521.
      For ab119521, the lysis buffer allows for lysis of the cellular membrane but not other compartments with in the cy...

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      Dear Sir, or Madam

      I would like to know if "Cytochrome c Human ELISA Kit (with color giving dyes) (ab119521)" is suitable for detecting cytochrome c levels in patients serum.

      Inside the product manual it is noted that " O...

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      Thank you for your email and your interest in our products.

      Unfortunately, we would not recommend using the Cytochrome c Human ELISA Kit (with color giving dyes), ab119521 with serum sample as when we have tried this we have observed false positi...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"