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Product name
Cytochrome c Protein Quantity Microplate Assay Kit
See all Cytochrome c products (2) ...
Tests
1 x 96 well plate
Sample type
Cell culture extracts, Tissue
Assay type
Sandwich
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Product overview
ab110172 (MSA41) is a 96-well microplate assay that is used to determine the amount of cytochrome c in a human, mouse, rat or bovine sample. Cytochrome c is immunocaptured within the wells and the amount is determined by adding a cytochrome c specific antibody conjugated with horseradish peroxidase. This peroxidase changes the substrate from colorless to blue at 600 nm. This reaction takes place in a time dependent manner proportional to the amount of protein captured in the wells. The rate of development of blue color can be followed at 600 nm or the reaction can be stopped at a user defined time by the addition of 100 µl of 1.5N HCl to each well (not supplied) and read at 450 nm. Included for the performance of this quantity assay are buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
Tested applications
Sandwich ELISAmore details
Properties
Storage instructions
Store at 4°C for upto 6 months. Refer to the protocol for further storage instructions.
| Components | Identifier | 96 tests |
|---|---|---|
| 10X Blocking Buffer | Tube 2 | 1 x 15ml |
| 20X Buffer | Tube 1 | 1 x 15ml |
| 20X Detector antibody | Tube A | 1 x 1ml |
| 20X HRP label | Tube B | 1 x 1ml |
| 20X Wash Buffer | Tube 3 | 1 x 15ml |
| 96-well pre-coated microplate | 1 unit | |
| Detergent | 1 x 1ml | |
| Development Solution | 1 x 20ml |
Research areas
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Cytochromes
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Integration of energy
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Kits/ Lysates/ Other >> Kits >> Apoptosis Kits >> Other Apoptosis Kits
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Sandwich ELISA - Cytochrome c Protein Quantity Microplate Assay Kit (ab110172)
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Relevance
Cytochrome c plays an important role in apoptosis. The protein is located in the space between the inner and outer mitochondrial membranes. An apoptotic stimulus triggers the release of cytochrome c from the mitochondria into cytosol where it binds to Apaf-1. The cytochrome c/Apaf-1 complex activates caspase-9, which then activates caspase-3 and other downstream caspases.
Cellular localization
Mitochondrial
Alternative names
- MSA41
Database links
- Entrez Gene: 54205 Human
- Omim: 123970 Human
- SwissProt: P99999 Human
- Unigene: 437060 Human
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
sELISA
Cytochrome c Protein Quantity Microplate Assay Kit images:
Sandwich ELISA - Cytochrome c Protein Quantity Microplate Assay Kit (ab110172)
Abcam's protein quantity microplate assays use the well-established sandwich ELISA format, whereby capture and detector antibodies are used to immobilize and then quantify a target protein or enzyme. All of our microplate assays utilize our highly-validated immunocapture antibodies, which are able to capture large, multi-subunit enzyme complexes in their fully intact state. Capture antibodies are pre-coated in the wells of premium Nunc MaxiSorp™ modular microplates, which can be broken into 8-well strips. After the target has been immobilized in the well, a second monoclonal antibody, against a different epitope on the target, is added to the well. This detector antibody is either directly labeled with biotin, or a biotin-labeled goat anti-mouse secondary is added. Substrate plus HRP or AP conjugated to streptavidin provide a colorimetric signal that is readable by any plate readers capable of standard ELISA absorbance measurements.
Protocols
References for Cytochrome c Protein Quantity Microplate Assay Kit (ab110172)
This product has been referenced in:
- Eliseev RA et al. Cyclophilin D interacts with Bcl2 and exerts an anti-apoptotic effect. J Biol Chem 284:9692-9 (2009).Read more (PubMed: 19228691) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"