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MitoSciences (MSA41)

Cytochrome c Protein Quantity Microplate Assay Kit (ab110172)

Q&A (10)Specific References (1)

Overview

  • Product nameCytochrome c Protein Quantity Microplate Assay KitSee all Cytochrome c kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts, Tissue
  • Assay typeSandwich
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Product overview

    ab110172 (MSA41) is a 96-well microplate assay that is used to determine the amount of cytochrome c in a human, mouse, rat or bovine sample. Cytochrome c is immunocaptured within the wells and the amount is determined by adding a cytochrome c specific antibody conjugated with horseradish peroxidase. This peroxidase changes the substrate from colorless to blue at 600 nm. This reaction takes place in a time dependent manner proportional to the amount of protein captured in the wells. The rate of development of blue color can be followed at 600 nm or the reaction can be stopped at a user defined time by the addition of 100 µl of 1.5N HCl to each well (not supplied) and read at 450 nm. Included for the performance of this quantity assay are buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

  • Tested applicationsSandwich ELISA more details

Properties

  • RelevanceCytochrome c plays an important role in apoptosis. The protein is located in the space between the inner and outer mitochondrial membranes. An apoptotic stimulus triggers the release of cytochrome c from the mitochondria into cytosol where it binds to Apaf-1. The cytochrome c/Apaf-1 complex activates caspase-9, which then activates caspase-3 and other downstream caspases.
  • Cellular localizationMitochondrial
    • Alternative names
        MSA41
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab110172 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Cytochrome c Protein Quantity Microplate Assay Kit images

      • Sandwich ELISA - Cytochrome c Protein Quantity Microplate Assay Kit (ab110172)
        Sandwich ELISA - Cytochrome c Protein Quantity Microplate Assay Kit (ab110172)
        Principle of Sandwich ELISA used in Cytochrome c Protein Quantity Microplate Assay Kit (ab110172). Substrate plus HRP or AP conjugated to streptavidin provide a colorimetric signal that is readable by any plate readers capable of standard ELISA absorbance measurements.

      Protocols

      References for Cytochrome c Protein Quantity Microplate Assay Kit (ab110172)

      This product has been referenced in:

      See 1 Publication for this product

      Publishing research using ab110172 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab110172 Submit a Question for ab110172

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      I'm looking again for your product ab110172 in order to measure enzymatic activity of Cytochrome C in C2C12 (myoblasts cell line), i see that i can't use this product because i haven't an idea about the spectrophotometer that you have recommanded, an othe...

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      For the kit ab110172, you will need a spectrophotometer that can measure at 450nm. These are generally available in most labs, so perhaps a neighboring lab has one? I really don't have a recommendation of a specific one to use, just as long as it can meas...

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      What is the detergent in this kit?

      Does the kit breaks the cell membrane as well as mitochondrial membrane?

      Thank you for contacting us.

      The Detergent supplied with MSA41 (ab110172) contains SDS. It will lyse all membranes including plasma membrane and mitochondrial membranes. Remember the purpose of this kit is to measure total cytochrome c concentrat...

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      What is expected length of time I can keep this kit at 4 degrees C?

      6 months at 4 degrees C is a reasonable length of storage.

      Does the insoluble fraction contain unreleased cytochrome C embedded I mitochondrial membrane?

      The question I have is if we are testing for total cytochrome C, why would the negative controls be any different than the positive controls? Or does...

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      Thank you for contacting us.

      The lab let me know that, if the sample is prepared according to the procedure in section C then:

      All cytochrome c, regardless its subcellular location, is extracted and present in the supernatant. Pellet ...

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      Thank you. I look forward to you opinion regarding the step 6.

      Thank you for your patience.

      In Chapter 6, section C describes extraction of total protein with Detergent. The great majority of proteins, including all cytochrome c are solubilized by the Detergent. The centrifugation step (step 6) is to clear...

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      uses cultured cells
      what does the kit measure? cytosolic, mitochondrial or total cytochrome C?
      page 13, step 6 - what is separated here? protocol unclear

      Thank you for contacting us.

      I have received the following information from the lab so far:

      This ELISA-based assaymeasures total cytochrome c in the sample of interest. These samples can be extracts of whole cultured cells and tissue ...

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      Hi, I have recently placed an order for the following kits:

      Ab109721

      Ab109875

      Ab110172

      Ab109911

      Ab109908

      Would you be able to let me know if these kits have been successfully used with prev...

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      Thank you for contacting us.
      These kits will be OK to use with snap frozen tissues.
      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
      Use our products? Submit an Abre...

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      What is the guarantee period for these kits?

      Thank you for contacting us.

      The kits are guaranteed for 6 months if stored as recommended on the datasheet.

      Please note there are components in the kits that are supposed to stored at different temperatures e.g. -20 or -80C, so we re...

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      can ab110172 be used to detect protein in serum samples (released from blood cells)?
      do we have other kits that would work with mitochondrial proteins in serum?

      Thank you for contacting us.

      Regarding ab110172 I received the following information from the lab:

      This kit can be used to test serum samples. Our suggestion for the customer would be to spike a control serum sample with pure cytochro...

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      Can this Cytochrome c kit be used on live cells?  If not, do you have any other products that could be used to quantify Cytochrome c released from live cells with induced apoptosis?

      Thank you for contacting us.  My colleague in the lab has confirmed that the Cytochrome c kit ab110172 is designed for use with cells or tissues prepared with a lysis buffer or a cell fractionation kit such as these:  http://www.abcam.com/Cell-Fractionati...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"