Anti-Cytokeratin 10 antibody [DE-K10] - BSA and Azide free (ab80523)
- Product nameAnti-Cytokeratin 10 antibody [DE-K10] - BSA and Azide freeSee all Cytokeratin 10 primary antibodies ...
- DescriptionMouse monoclonal [DE-K10] to Cytokeratin 10 - BSA and Azide free
- Tested applicationsWB, IHC-P, ICC/IF more details
- Species reactivityReacts with: Rat, Cat, Dog, Human
Cytoskeletal preparation extracted from Human ectocervical epithelium.
- Positive controlA431 cells. Skin.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 10mM PBS, pH 7.4
- Concentration information loading...
- PurityProtein G purified
- Clonality Monoclonal
- Clone numberDE-K10
- Research Areas
Our Abpromise guarantee covers the use of ab80523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at a concentration of 0.5 - 1 µg/ml. Antigen retrieval is not required.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- Tissue specificitySeen in all suprabasal cell layers including stratum corneum.
- Involvement in diseaseDefects in KRT10 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
Defects in KRT10 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.
- Sequence similaritiesBelongs to the intermediate filament family.
- 56 kDa cytokeratin antibodyBCIE antibodyBIE antibody
- CK 10 antibodyCK-10 antibodyck10 antibodyCytokeratin 10 antibodyCytokeratin 6B antibodyCytokeratin VIB antibodyCytokeratin-10 antibodyCytokeratin10 antibodyEHK antibodyk10 antibodyK1C10_HUMAN antibodyKeratin 10 antibodyKeratin antibodyKeratin type i cytoskeletal 10 antibodyKeratin type I cytoskeletal 59 kDa antibodyKeratin-10 antibodyKeratin10 antibodykpp antibodyKrt 1 10 antibodyKrt 10 antibodyKRT10 antibodykrt10 antibodytype I cytoskeletal 10 antibodyType I keratin Ka10 antibody
Anti-Cytokeratin 10 antibody [DE-K10] - BSA and Azide free images
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human skin tissue using 1µg/ml ab80519 in with a peroxidase-conjugated secondary antibody and an AEC chromogen. Note cytoplasmic staining of epidermal region.
Anti-Cytokeratin 10 antibody [DE-K10] - BSA and Azide free (ab80523) at 2 µg/ml + A431 cell lysate
Predicted band size : 60 kDa
Observed band size : 60 kDa
References for Anti-Cytokeratin 10 antibody [DE-K10] - BSA and Azide free (ab80523)
ab80523 has not yet been referenced specifically in any publications.