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Anti-Cytokeratin 10 antibody [DE-K10] - BSA and Azide free (ab80523)

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Overview

  • Product nameAnti-Cytokeratin 10 antibody [DE-K10] - BSA and Azide freeSee all Cytokeratin 10 primary antibodies ...
  • Description
    Mouse monoclonal [DE-K10] to Cytokeratin 10 - BSA and Azide free
  • Tested applicationsWB, IHC-P, ICC/IF more details
  • Species reactivity
    Reacts with: Rat, Cat, Dog, Human
  • Immunogen

    Cytoskeletal preparation extracted from Human ectocervical epithelium.

  • Positive controlA431 cells. Skin.

Properties

Applications

Our Abpromise guarantee covers the use of ab80523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IHC-P
ICC/IF
  • Application notesICC/IF: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 0.5 - 1 µg/ml. Antigen retrieval is not required.
    WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Tissue specificitySeen in all suprabasal cell layers including stratum corneum.
    • Involvement in diseaseDefects in KRT10 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
      Defects in KRT10 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.
    • Sequence similaritiesBelongs to the intermediate filament family.

      • Target information above from: UniProt accession P13645 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          56 kDa cytokeratin antibodyBCIE antibodyBIE antibody
          CK 10 antibodyCK-10 antibodyck10 antibodyCytokeratin 10 antibodyCytokeratin 6B antibodyCytokeratin VIB antibodyCytokeratin-10 antibodyCytokeratin10 antibodyEHK antibodyk10 antibodyK1C10_HUMAN antibodyKeratin 10 antibodyKeratin antibodyKeratin type i cytoskeletal 10 antibodyKeratin type I cytoskeletal 59 kDa antibodyKeratin-10 antibodyKeratin10 antibodykpp antibodyKrt 1 10 antibodyKrt 10 antibodyKRT10 antibodykrt10  antibodytype I cytoskeletal 10 antibodyType I keratin Ka10 antibody
        see all

      Anti-Cytokeratin 10 antibody [DE-K10] - BSA and Azide free images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 10 antibody [DE-K10] - BSA and Azide free (ab80523)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 10 antibody [DE-K10] - BSA and Azide free (ab80523)
        Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human skin tissue using 1µg/ml ab80519 in with a peroxidase-conjugated secondary antibody and an AEC chromogen. Note cytoplasmic staining of epidermal region.
      • Western blot - Cytokeratin 10 antibody [DE-K10] - BSA and Azide free (ab80523)
        Western blot - Cytokeratin 10 antibody [DE-K10] - BSA and Azide free (ab80523)
        Anti-Cytokeratin 10 antibody [DE-K10] - BSA and Azide free (ab80523) at 2 µg/ml + A431 cell lysate

        Predicted band size : 60 kDa
        Observed band size : 60 kDa

      References for Anti-Cytokeratin 10 antibody [DE-K10] - BSA and Azide free (ab80523)

      ab80523 has not yet been referenced specifically in any publications.

      Publishing research using ab80523 ? Please let us know so that we can cite the reference in this datasheet

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