Anti-Cytokeratin 10 antibody [EP1607IHCY] (ab76318)

Overview

• Product nameAnti-Cytokeratin 10 antibody [EP1607IHCY]See all Cytokeratin 10 primary antibodies ...
• Description
  Rabbit monoclonal [EP1607IHCY] to Cytokeratin 10
• SpecificitySome customers have successfully used ab76318 to staining Cytokeratin 10 in mouse tissue. Please see Abreviews for more details.
• Tested applicationsWB, IP, IHC-P, ICC more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide corresponding to residues in the C-terminal of human Cytokeratin 10.

• Positive controlHaCat cell lysate. Human skin tissue.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEP1607IHCY
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Intermediate Filaments
  • Class I
  • Cytokeratins

Applications

Target

• Tissue specificitySeen in all suprabasal cell layers including stratum corneum.
• Involvement in diseaseDefects in KRT10 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
  Defects in KRT10 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.
• Sequence similaritiesBelongs to the intermediate filament family.

Target information above from: UniProt accession P13645 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3858 Human
  • Entrez Gene: 16661 Mouse
  • Entrez Gene: 450225 Rat
  • Omim: 148080 Human
  • SwissProt: P13645 Human
  • SwissProt: P02535 Mouse
  • SwissProt: Q6IFW6 Rat
  • Unigene: 99936 Human

  • Unigene: 22662 Mouse
  • Unigene: 125065 Rat

  see all

• Alternative names
  56 kDa cytokeratin antibodyBCIE antibodyBIE antibody

  CK 10 antibodyCK-10 antibodyck10 antibodyCytokeratin 10 antibodyCytokeratin 6B antibodyCytokeratin VIB antibodyCytokeratin-10 antibodyCytokeratin10 antibodyEHK antibodyk10 antibodyK1C10_HUMAN antibodyKeratin 10 antibodyKeratin antibodyKeratin type i cytoskeletal 10 antibodyKeratin type I cytoskeletal 59 kDa antibodyKeratin-10 antibodyKeratin10 antibodykpp antibodyKrt 1 10 antibodyKrt 10 antibodyKRT10 antibodykrt10  antibodytype I cytoskeletal 10 antibodyType I keratin Ka10 antibody

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Anti-Cytokeratin 10 antibody [EP1607IHCY] images

• 

  Western blot - Cytokeratin 10 antibody [EP1607IHCY] (ab76318)
  Anti-Cytokeratin 10 antibody [EP1607IHCY] (ab76318) at 1/100000 dilution + HaCat cell lysate at 10 µg
  
  Secondary
  Goat anti-rabbit HRP at 1/2000 dilution
  
  Predicted band size : 60 kDa
  Observed band size : 60 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 10 antibody [EP1607IHCY] (ab76318)
  ab76318, at 1/100-1/250 dilution, staining Cytokeratin 10 in paraffin-embedded human skin tissue by Immunohistochemistry. Note positive staining of suprabasal layers of keratinising stratified epithelia.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] (ab76318)
  Fluorescent immunohistochemical analysis of paraffin-embedded human normal skin tissue using ab76318. Green-CK10 red-PI
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] (ab76318)Carl Hobbs, King`s College London, United Kingdom
  IHC-P image of Cytokerati 10 staining on human skin section using ab76318 (1:6000). The sections were subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA for 10 mins at 21°C. ab76318 was incubated for 2 hours at 21°C. The secondary antibody used was Goat polyclonal to rabbit IgG conjugated to biotin (1:200).

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] (ab76318)Carl Hobbs, King`s College London, United Kingdom
  IHC-P image of Cytokerati 10 staining on rat skin section using ab76318 (1:10000). The sections were subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA for 10 mins at 21°C. ab76318 was incubated for 2 hours at 21°C. The secondary antibody used was Goat polyclonal to rabbit IgG conjugated to biotin (1:250).

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] (ab76318)Carl Hobbs, King`s College London, United Kingdom
  IHC-P image of Cytokerati 10 staining on mouse skin section using ab76318 (1:10000). The sections were subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA for 10 mins at 21°C. ab76318 was incubated for 2 hours at 21°C. The secondary antibody used was Goat polyclonal to rabbit IgG conjugated to biotin (1:250).

  See Abreview