Anti-Cytokeratin 10 antibody [RKSE60] (ab9025)
- Product nameAnti-Cytokeratin 10 antibody [RKSE60]See all Cytokeratin 10 primary antibodies ...
- DescriptionMouse monoclonal [RKSE60] to Cytokeratin 10
- SpecificityThis antibody reacts exclusively with cytokeratin 10 which is present in keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas
- Tested applicationsFlow Cyt, IHC-Fr, ICC, WB more details
- Species reactivityReacts with: Mouse, Rat, Dog, Human, Pig
Does not react withZebrafish
Cytokeratins from the human epidermis.
- Positive control
- human keratinocytes
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
- Concentration information loading...
- PurityProtein G purified
- Clonality Monoclonal
- Clone numberRKSE60
- Research Areas
Our Abpromise guarantee covers the use of ab9025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Flow Cyt: 1/100 - 1/200.|
|IHC-Fr||IHC-Fr: Use at an assay dependent dilution.|
|ICC||ICC: Use at an assay dependent dilution.|
|WB||WB: 1/500 - 1/1000. (see reviews). Detects two bands of approximately 55 kDa (heterotetramer of two type I and two type II keratins).|
- Tissue specificitySeen in all suprabasal cell layers including stratum corneum.
- Involvement in diseaseDefects in KRT10 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
Defects in KRT10 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.
- Sequence similaritiesBelongs to the intermediate filament family.
- BCIE antibody
- BIE antibody
- CK 10 antibody
- CK-10 antibody
- ck10 antibody
- Cytokeratin 10 antibody
- Cytokeratin-10 antibody
- Cytokeratin10 antibody
- EHK antibody
- k10 antibody
- K1C10_HUMAN antibody
- Keratin 10 antibody
- Keratin antibody
- Keratin type i cytoskeletal 10 antibody
- Keratin type I cytoskeletal 59 kDa antibody
- Keratin-10 antibody
- Keratin10 antibody
- kpp antibody
- Krt 10 antibody
- KRT10 antibody
- krt10 antibody
- type I cytoskeletal 10 antibody
Anti-Cytokeratin 10 antibody [RKSE60] images
ab9025 staining Cytokeratin 10 in human skin tissue by Immunohistochemistry (Frozen sections). The tissue was acetone fixed and then blocked using 0.25% casein for 10 minutes at 20°C. Samples were then incubated with primary antibody at 1/1000 for 1 hour at 20°C. The secondary antibody used was a mouse anti-mouse monoclonal conjugated to Alexa Fluor® 488 (green) used at a 1/200 dilution.
Immunohistochemistry on frozen section of swine skin: positive staining of the keratinizing layer
Immunohistochemistry on paraffin section of human skin
Immunofluorescence staining of a 7 days old zebrafish embryo
References for Anti-Cytokeratin 10 antibody [RKSE60] (ab9025)
This product has been referenced in:
- Li D et al. Lack of plakoglobin in epidermis leads to keratoderma. J Biol Chem 287:10435-43 (2012). ICC/IF ; Mouse . Read more (PubMed: 22315228) »
- Kandyba EE et al. A murine living skin equivalent amenable to live-cell imaging: analysis of the roles of connexins in the epidermis. J Invest Dermatol 128:1039-49 (2008). ICC/IF ; Mouse . Read more (PubMed: 17960178) »