Anti-Cytokeratin 10 antibody [RKSE60] (ab9025)

Overview

  • Product nameAnti-Cytokeratin 10 antibody [RKSE60]See all Cytokeratin 10 primary antibodies ...
  • Description
    Mouse monoclonal [RKSE60] to Cytokeratin 10
  • SpecificityThis antibody reacts exclusively with cytokeratin 10 which is present in keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas
  • Tested applicationsFlow Cyt, IHC-Fr, ICC, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human, Pig

    Does not react with

    Zebrafish
  • Immunogen

    Cytokeratins from the human epidermis.

  • Positive control
    • human keratinocytes

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Clonality Monoclonal
  • Clone numberRKSE60
  • MyelomaSp2/0
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab9025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100 - 1/200.
IHC-Fr Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
WB 1/500 - 1/1000. (see reviews). Detects two bands of approximately 55 kDa (heterotetramer of two type I and two type II keratins).

Target

  • Tissue specificitySeen in all suprabasal cell layers including stratum corneum.
  • Involvement in diseaseDefects in KRT10 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
    Defects in KRT10 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Information by UniProt
  • Database links
  • Alternative names
    • BCIE antibody
    • BIE antibody
    • CK 10 antibody
    • CK-10 antibody
    • ck10 antibody
    • Cytokeratin 10 antibody
    • Cytokeratin-10 antibody
    • Cytokeratin10 antibody
    • EHK antibody
    • k10 antibody
    • K1C10_HUMAN antibody
    • Keratin 10 antibody
    • Keratin antibody
    • Keratin type i cytoskeletal 10 antibody
    • Keratin type I cytoskeletal 59 kDa antibody
    • Keratin-10 antibody
    • Keratin10 antibody
    • kpp antibody
    • Krt 10 antibody
    • KRT10 antibody
    • krt10  antibody
    • type I cytoskeletal 10 antibody
    see all

Anti-Cytokeratin 10 antibody [RKSE60] images

  • ab9025 staining Cytokeratin 10 in human skin tissue by Immunohistochemistry (Frozen sections). The tissue was acetone fixed and then blocked using 0.25% casein for 10 minutes at 20°C. Samples were then incubated with primary antibody at 1/1000 for 1 hour at 20°C. The secondary antibody used was a mouse anti-mouse monoclonal conjugated to Alexa Fluor® 488 (green) used at a 1/200 dilution.

    See Abreview

  • Immunohistochemistry on frozen section of swine skin: positive staining of the keratinizing layer
  • Immunohistochemistry on paraffin section of human skin
  • Immunofluorescence staining of a 7 days old zebrafish embryo

References for Anti-Cytokeratin 10 antibody [RKSE60] (ab9025)

This product has been referenced in:
  • Li D  et al. Lack of plakoglobin in epidermis leads to keratoderma. J Biol Chem 287:10435-43 (2012). ICC/IF ; Mouse . Read more (PubMed: 22315228) »
  • Kandyba EE  et al. A murine living skin equivalent amenable to live-cell imaging: analysis of the roles of connexins in the epidermis. J Invest Dermatol 128:1039-49 (2008). ICC/IF ; Mouse . Read more (PubMed: 17960178) »

See all 6 Publications for this product

Product Wall

Vous avez testé l'anticorps anti-cytokeratin 10 et l'isotype en Western Blot. Avez-vous également utilisé l'anticorps secondaire seul?
Je suis étonné que la bande observée avec l'isotype se retrouve au m&ec...

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Mercipour votre réponse ainsi que pour le questionnaire rempli et détaillé.

Il m'est toujours impossible de trouver une explication définitive à votre problème.
Est-ce que l'anticorps anti-K10 est ...

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En plus de vos résultats obtenus en Western Blot, vous serait-il possible de remplir et nous retourner le questionnaire ci-joint?

Les informations sur le protocole utilisé nous sera utile pour tenter de comprendre le problè...

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Merci de nous avoir contactés.

Je vous envoie comme convenu un email auquel vous pourrez répondre et joindre vos résultats en Western Blot avec les anticorps ab9025 et ab18448.
Votre réponse me sera alors automa...

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Le laboratoire vient de nous confirmer que la chaine légère du clone [RKSE60] est de type kappa.

Thank you for your phone call. I found just the information in our records and am happy to confirm that the laboratory has obtained good results with this antibody at 1:100 – 1:200 for flow cytometry. I hope this information is helpful. Please do no...

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Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Skin)
Specification Skin
Fixative Acetone
Permeabilization No
Blocking step Casein as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: 20°C
Username

Dr. Vaughan Feisst

Verified customer

Submitted Sep 17 2010

Thank you for contacting us for technical support. I do not advise the customer to use this antibody on mouse tissue in immunohistochemical methods as it is raised in mouse. Therefore the secondary anti-mouse antibody will bind to the whole of the t...

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Thank you for the details that you have provided and I'm sorry to hear that you are still experiencing difficulty with ab9025. I don't see any problems with your protocol and you have tried several variations to trouble-shoot. I can offer to send you a...

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Thank you for contacting us. As discussed over the phone, pleaes find the link to our technical support questionaire below. If you could fill this out and submit it, we would be happy to look into the issue further. http://www.abcam.com/index.html...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"