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Cytokeratin 14 protein (Human) (ab73637)

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Overview

  • Product nameCytokeratin 14 protein (Human)
  • Protein description Recombinant Full Length Human Cytokeratin 14, 51,530 Dalton. Swiss ID = P02533.
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesab73637 is purified by proprietary chromatographic techniques. Purity is greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
  • FormLyophilised:Reconstitute in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 9.5M Urea, 30mM Tris HCl, 2mM EDTA, 10mM methylammonium chloride, pH 8
  • Concentration information loading...
  • Additional notesReconstitution to filaments: Performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM NaCI, 2mM dithiothreitol, 10mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab73637 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    WB WB: Use at an assay dependent concentration. Ab73637 can be used as a WB positive control in conjunction with ab7800.

    Protein info

    • Alternative names
        CK 14CK-14ck14
        Cytokeratin 14Cytokeratin-14Cytokeratin14Dowling Mearaebs3ebs4Epidermolysis bullosa simplexk14K1C14_HUMANKeratinKeratin 14Keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)Keratin type I cytoskeletal 14Keratin-14Keratin14KoebnerKrt 14krt14NFJOTTHUMP00000164624type I cytoskeletal 14
      see all
  • FunctionThe nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
  • Tissue specificityDetected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
  • Involvement in diseaseDefects in KRT14 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
    Defects in KRT14 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.
    Defects in KRT14 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, although it is less severe.
    Defects in KRT14 are the cause of epidermolysis bullosa simplex autosomal recessive (AREBS) [MIM:601001]. AREBS is an intraepidermal epidermolysis bullosa characterized by localized blistering on the dorsal, lateral and plantar surfaces of the feet.
    Defects in KRT14 are the cause of Naegeli-Franceschetti-Jadassohn syndrome (NFJS) [MIM:161000]; also known as Naegeli syndrome. NFJS is a rare autosomal dominant form of ectodermal dysplasia. The cardinal features are absence of dermatoglyphics (fingerprints), reticular cutaneous hyperpigmentation (starting at about the age of 2 years without a preceding inflammatory stage), palmoplantar keratoderma, hypohidrosis with diminished sweat gland function and discomfort provoked by heat, nail dystrophy, and tooth enamel defects.
    Defects in KRT14 are the cause of dermatopathia pigmentosa reticularis (DPR) [MIM:125595]. DPR is a rare ectodermal dysplasia characterized by lifelong persistent reticulate hyperpigmentation, noncicatricial alopecia, and nail dystrophy.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Cellular localizationCytoplasm. Nucleus. Expressed in both as a filamentous pattern.

    • Target information above from: UniProt accession P02533 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Cytokeratin 14 protein (Human) images

    • Western blot - Anti-Cytokeratin 14 antibody [LL002] (ab7800)
      Western blot - Anti-Cytokeratin 14 antibody [LL002] (ab7800)
      All lanes : Anti-Cytokeratin 14 antibody [LL002] (ab7800) at 2 µg/ml

      Lane 1 : Cytokeratin 14 protein (Human) (ab73637) at 0.1 µg
      Lane 2 : Cytokeratin 14 protein (Human) (ab73637) at 0.01 µg

      Secondary
      Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 10 seconds

    References for Cytokeratin 14 protein (Human) (ab73637)

    ab73637 has not yet been referenced specifically in any publications.

    Publishing research using ab73637 ? Please let us know so that we can cite the reference in this datasheet

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    Thank you very much for your information. I will work on it in the next couple of days. Could you please help me to see if you have Keratin 14 antibody? I want it to react to human and mouse cells. Also, please check if you have protein or peptide for it ...

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    Thank you for your reply.

    For Cytokeratin 14, I would recommend the rabbit polyclonal antibody ab48937. This antibody is validated for use in ELISA on human samples, and is predicted to react with mouse. Since this antibody has not been validate...

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