Cytokeratin 18 antibody [B23.1. (DC 10)] (FITC) (ab7798)

Product Name 

Cytokeratin 18 antibody [B23.1. (DC 10)] (FITC)

Product type 

Primary antibodies

Description 

Mouse monoclonal [B23.1. (DC 10)] to Cytokeratin 18 (FITC)

Immunogen 

PMC-42 human breast carcinoma cells.

Reacts with 
(species key)

Hu

Specificity 

Specific reactivity for the cytokeratin 18 (45 kD) polypeptide.

Tested applications 
(see key)

Flow Cyt, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Flow cytometry 1-5 ug / 10 6 cells.
Immunoblotting 1-5 ug / mL.
Immunohistochemistry 5-10 ug / mL on frozen sections using avidin-biotin system.
Immunofluorescence (10 - 20 ug / mL) on paraffin-embedded sections, frozen sections or cell smears.
Not tested in other applications.

Optimal dilutions / concentrations should be determined by the end user.

The antibody may be usedfor 2 hours at room temperature on formalin-fixed, paraffin-embedded tissue sections.

When using paraffin-embeddedand formalin-fixed tissues, predigestion or high temperature antigenicunmasking is strongly recommended for consistent and reproducible results.

Positive control 
(see definition)

Colon carcinoma

Cellular localization 

Cytoplasmic

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated

Relevance 

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non keratinizing cells (ie. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.

Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.

This antibody reacts with a wide variety of simple epithelia.
It does not react with stratified squamous epithelia.
It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary and thyroid.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

B23.1. (DC 10)

Isotype 

IgG1

Purity 

IgG fraction

Conjugation notes 

The antibody is conjugated with FITC at a ratio of 6 moles of Fluorescein to 1 mole of antibody.
Maximum excitation of FITC occurs at wavelength 492 nm, and maximum emission occurs at wavelength 520 nm.

Storage buffer 

n/a

Form 

Liquid

Conjugation 

Fluorescein

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C in the dark. Do not freeze.

See below for Cytokeratin 18 antibody [B23.1. (DC 10)] (FITC) images, references, products related to ab7798 and other tools.

Cytokeratin 18 antibody [B23.1. (DC 10)] (FITC) images:

References for Cytokeratin 18 antibody [B23.1. (DC 10)] (FITC) (ab7798)

  This product has been used in: (two most recent references)

Yin L et al. ABCG2 expression and side population abundance regulated by a transforming growth factor beta-directed epithelial-mesenchymal transition. Cancer Res 68:800-7 (2008). Flow Cyt; Human. PubMed: 18245481

Skibinski G et al. Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor. Am J Physiol Lung Cell Mol Physiol 293:L69-76 (2007). PubMed: 17384084
If you publish research using ab7798 please let us know so that we can cite the reference on this datasheet.

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