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Immunocytochemistry/ Immunofluorescence - Cytokeratin 18 antibody [C-04] (ab668)
![Immunocytochemistry/ Immunofluorescence - Cytokeratin 18 antibody [C-04] (ab668)](/ps/datasheet/images/ab668_2.jpg)
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Product Name
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Cytokeratin 18 antibody [C-04]
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See all Cytokeratin 18 antibodies (31)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [C-04] to Cytokeratin 18
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Immunogen
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Tissue / cell preparation (Human). (Cytoskeleton preparation of epidermal carcinoma cell line A431).
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Reacts with
(species key)
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Hu, Ms, Rat, Cow, Dog, Hm, Pig, Shp
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Specificity
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Human Cytokeratin
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use at an assay dependent dilution (PMID 18946470).
ICC: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent concentration.
IHC-P: Use at an assay dependent dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 45 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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A431 cells
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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Relevance
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Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non keratinizing cells (ie. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.
Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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C-04
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Isotype
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IgG1
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from culture supernatant using protein A-affinity chromatography.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cytokeratin 18 antibody [C-04] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Cytokeratin 18 antibody [C-04] images, references, products related to ab668 and other tools.
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Cytokeratin 18 antibody [C-04] images:
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 Immunocytochemistry/ Immunofluorescence - Cytokeratin 18 antibody [C-04] (ab668)
ab668 at 1/100 staining A431 Human epithelial carcinoma cells by ICC/IF. The cells were paraformaldehyde fixed, permeabilized with 0.25% Triton X-100 and then blocked with BSA prior to incubation with the antibody for 24 hours at 4°C. A FITC conjugated goat anti-mouse IgG antibody was used as the secondary. The right hand image shows staining with ab668 followed by secondary antibody. The left hand image shows DAPI staining with secondary only. This image is courtesy of an anonymous Abreview
See Abreview
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References for Cytokeratin 18 antibody [C-04] (ab668)
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This product has been used in: (two most recent references)
Goossens K et al. Suppression of keratin 18 gene expression in bovine blastocysts by RNA interference. Reprod Fertil Dev 22:395-404 (2010). ICC/IF; Cow. PubMed: 20047725
Klinakis A et al. Igf1r as a therapeutic target in a mouse model of basal-like breast cancer. Proc Natl Acad Sci U S A 106:2359-64 (2009). IHC-P; Mouse. PubMed: 19174523
See all 17 publication references for this product.
If you publish research using ab668 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Cytokeratin 18
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Cytokeratin 18 antibody [C-04] - more information
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunocytochemistry/ Immunofluorescence - Cytokeratin 18 antibody [C-04] (ab668)](/index.html?datasheet=668#ab668_2.jpg)
