Anti-Cytokeratin 18 antibody [DC-10] (FITC) (ab72813)

Overview

• Product nameAnti-Cytokeratin 18 antibody [DC-10] (FITC)See all Cytokeratin 18 primary antibodies ...
• Description
  Mouse monoclonal [DC-10] to Cytokeratin 18 (FITC)
• ConjugationFITC
• Tested applicationsFlow Cyt, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Human breast carcinoma cell line PMC-42.

• Positive controlIF/ICC: HCT116 cell line.

Properties

• FormLiquid
• Storage instructionsStore at +4°C in the dark. Do not freeze.
• Storage bufferPreservative: 0.097% Sodium azide
  Constituent: PBS
• Concentration information loading...
• PuritySize exclusion
• Purification notesThe purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
• Clonality Monoclonal
• Clone numberDC-10
• IsotypeIgG1
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Intermediate Filaments
  • Class I
  • Cytokeratins

  • Tags & Cell Markers
  • Cell Type Markers
  • Tumor Associated

Applications

Target

• FunctionInvolved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
• Tissue specificityExpressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
• Involvement in diseaseDefects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
• Sequence similaritiesBelongs to the intermediate filament family.
• Post-translational
  modificationsPhosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
  Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
  O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
• Cellular localizationCytoplasm > perinuclear region.

Target information above from: UniProt accession P05783 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3875 Human
  • Omim: 148070 Human
  • SwissProt: P05783 Human
  • Unigene: 406013 Human

• Alternative names
  Cell proliferation inducing gene 46 protein antibodyCell proliferation inducing protein 46 antibodyCell proliferation-inducing gene 46 protein antibody

  CK 18 antibodyCK-18 antibodyCK18 antibodyCYK 18 antibodyCYK18 antibodyCytokeratin 18 antibodyCytokeratin endo B antibodyCytokeratin-18 antibodyCytokeratin18 antibodyK 18 antibodyK18 antibodyK1C18_HUMAN antibodyKeratin 18 antibodyKeratin D antibodyKeratin type I cytoskeletal 18 antibodykeratin, type I cytoskeletal 18 antibodyKeratin-18 antibodyKeratin18 antibodyKerd antibodyKRT18 antibody

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Anti-Cytokeratin 18 antibody [DC-10] (FITC) images

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  Flow Cytometry-Anti-Cytokeratin 18 antibody [DC-10] (FITC)(ab72813)
  Overlay histogram showing MCF-7 cells stained with ab72813 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab72813, 0.5µg/1x10⁶ cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (FITC) (2µg/1x10⁶ cells) for 30 min at 22°C. Acquisition of >5,000 events was performed.
  
  
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  Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [DC-10] (FITC) (ab72813)
  ICC/IF image of ab72813 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72813, 1µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.