Anti-Cytokeratin 18 antibody [DC-10] (FITC) (ab72813)

Overview

  • Product nameAnti-Cytokeratin 18 antibody [DC-10] (FITC)
    See all Cytokeratin 18 primary antibodies
  • Description
    Mouse monoclonal [DC-10] to Cytokeratin 18 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsFlow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human breast carcinoma cell line PMC-42.

  • Positive control
    • IF/ICC: HCT116 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab72813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 0.5 - 1 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionInvolved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • Tissue specificityExpressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • Involvement in diseaseDefects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    modifications
    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • Cellular localizationCytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • Cytokeratin18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • Keratin 18 antibody
    • Keratin D antibody
    • Keratin type I cytoskeletal 18 antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Keratin18 antibody
    • Kerd antibody
    • KRT18 antibody
    see all

Anti-Cytokeratin 18 antibody [DC-10] (FITC) images

  • Overlay histogram showing MCF-7 cells stained with ab72813 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab72813, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (FITC) (2µg/1x106 cells) for 30 min at 22°C. Acquisition of >5,000 events was performed.

  • ICC/IF image of ab72813 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72813, 1µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Cytokeratin 18 antibody [DC-10] (FITC) (ab72813)

ab72813 has not yet been referenced specifically in any publications.

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