Anti-Cytokeratin 18 antibody [DC10] (ab231431)
Key features and details
- Mouse monoclonal [DC10] to Cytokeratin 18
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Cytokeratin 18 antibody [DC10]
See all Cytokeratin 18 primary antibodies -
Description
Mouse monoclonal [DC10] to Cytokeratin 18 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human Cytokeratin 18. Human breast cancer PMC42 cells.
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Positive control
- IHC-P: Human breast carcinoma, breast, ductal carcinoma, hepatocellular carcinoma, ductal carcinoma, prostate, prostate adenocarcinoma, colon, colon adenocarcinoma, bladder, bladder transitional cell carcinoma and liver tissues. WB: A431 cell lysate.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by Protein A/G. -
Clonality
Monoclonal -
Clone number
DC10 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100.
Boil tissue section in Citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 30 minutes at room temperature. |
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WB |
1/25. Predicted molecular weight: 48 kDa.
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Notes |
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IHC-P
1/100. Boil tissue section in Citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 30 minutes at room temperature. |
WB
1/25. Predicted molecular weight: 48 kDa. |
Target
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Function
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. -
Tissue specificity
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. -
Involvement in disease
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600]. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsPhosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. -
Cellular localization
Cytoplasm > perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 3875 Human
- Omim: 148070 Human
- SwissProt: P05783 Human
- Unigene: 406013 Human
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Alternative names
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
- Cell proliferation-inducing gene 46 protein antibody
see all
Images
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Anti-Cytokeratin 18 antibody [DC10] (ab231431) at 1/25 dilution + A431 (human epidermoid carcinoma cell line) cell lysate.
Developed using the ECL technique.
Predicted band size: 48 kDa -
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Cytokeratin 18 using ab231431 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human breast tissue stained for Cytokeratin 18 using ab231431 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Cytokeratin 18 using ab231431 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for Cytokeratin 18 using ab231431 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Cytokeratin 18 using ab231431 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human prostate tissue stained for Cytokeratin 18 using ab231431 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human prostate Adenocarcinoma tissue stained for Cytokeratin 18 using ab231431 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon tissue stained for Cytokeratin 18 using ab231431 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon Adenocarcinoma tissue stained for Cytokeratin 18 using ab231431 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human bladder tissue stained for Cytokeratin 18 using ab231431 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for Cytokeratin 18 using ab231431 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human liver tissue stained for Cytokeratin 18 using ab231431 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231431 has not yet been referenced specifically in any publications.