Cytokeratin 18 antibody [DEK-18] (ab9387)

Product Name 

Cytokeratin 18 antibody [DEK-18]

Product type 

Primary antibodies

Description 

Mouse monoclonal [DEK-18] to Cytokeratin 18

Immunogen 

Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431

Reacts with 
(species key)

Hu, Cat, Dog

Specificity 

This antibody reacts exclusively with cytokeratin 18

Tested applications 
(see key)

Flow Cyt, ICC, IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/100 - 1/200.
ICC: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
For staining on paraffin-embedded tissues pretreatment with 0.1% pepsin in 0.1 N HCl 30 min at room temperature is required.
Recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
WB: Use at a concentration of 0.1 - 0.2 µg/ml. Perform Western blot of cytoskeletal protein extract.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Cytoskeletal preparation of HaCaT cells.

Cellular localization 

Cytoplasmic

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated

Relevance 

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non keratinizing cells (ie. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.

Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

DEK-18

Myeloma 

Sp2/0

Isotype 

IgG1

Light chain type 

kappa

Purity 

Protein G purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Cytokeratin 18 antibody [DEK-18] images, references, products related to ab9387 and other tools.

Cytokeratin 18 antibody [DEK-18] images:

References for Cytokeratin 18 antibody [DEK-18] (ab9387)

  This product has been used in:

Glebov OO et al. Flotillin-1 defines a clathrin-independent endocytic pathway in mammalian cells. Nat Cell Biol 8:46-54 (2006). PubMed: 16341206
If you publish research using ab9387 please let us know so that we can cite the reference on this datasheet.

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