![Western blot - Cytokeratin 18 antibody [DEK-18] (ab9387) image](#ab9387_1.jpg)
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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Overview
Product name
Anti-Cytokeratin 18 antibody [DEK-18]
See all Cytokeratin 18 products (35) ...
Description
Mouse monoclonal [DEK-18] to Cytokeratin 18
Specificity
This antibody reacts exclusively with cytokeratin 18
Tested applications
IHC-P, WB, IHC-Fr, ICC, Flow Cytmore details
Cross reactivity
Reacts with
Cat, Dog, Human
Immunogen
Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431
Positive control
Cytoskeletal preparation of HaCaT cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
DEK-18
Myeloma
Sp2/0
Isotype
IgG1
Light chain type
kappa
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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Western blot - Cytokeratin 18 antibody [DEK-18] (ab9387)
![Western blot - Cytokeratin 18 antibody [DEK-18] (ab9387) image](/ps/datasheet/Images/9/ab9387/ab9387_1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab9387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/200.Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
WB: Use a concentration of 0.1 - 0.2 µg/ml.
IHC-Fr: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
Flow Cyt
Flow Cyt: 1/100 - 1/200.Flow Cyt: 1/100 - 1/200.
Target
Function
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Tissue specificity
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
Involvement in disease
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
Sequence similarities
Belongs to the intermediate filament family.
Post-translational
modifications
Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
Cellular localization
Cytoplasm > perinuclear region.
Target information above from: UniProt accessionP05783
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- type I cytoskeletal 18 antibody
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
see all
Database links
- Entrez Gene: 477601 Dog
- Entrez Gene: 3875 Human
- Omim: 148070 Human
- SwissProt: P05783 Human
- Unigene: 18481 Dog
- Unigene: 406013 Human
- Unigene: 598127 Human
Anti-Cytokeratin 18 antibody [DEK-18] images:
Western blot - Cytokeratin 18 antibody [DEK-18] (ab9387)
Western blot performed with ab9387 on 1.5 ul of cytoskeletal preparation from HaCaT cells.
References for Anti-Cytokeratin 18 antibody [DEK-18] (ab9387)
This product has been referenced in:
- Glebov OOet al. Flotillin-1 defines a clathrin-independent endocytic pathway in mammalian cells. Nat Cell Biol 8:46-54 (2006).Read more (PubMed: 16341206) »
See 1 publication for this product
Publishing research using ab9387? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"