Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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ab32118 has been referenced in 5 publications.
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Anti-Cytokeratin 18 antibody [E431-1] (ab32118) at 1/10000 dilution + A431 cell lysate
Predicted band size : 48 kDa
Observed band size : 48 kDa
Ab32118, at a dilution of 1/100, staining Cytokeratin 18 in paraffin embedded colon carcinoma tissue sections by Immunohistochemistry.
ICC/IF image of ab32118 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32118, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab32118 showing positive staining in Breast carcinoma tissue.
ab32118 showing positive staining in Gastric adenocarcinoma tissue.
ab32118 showing positive staining in Normal colon tissue.
ab32118 showing positive staining in Normal kidney tissue.
ab32118 showing negative staining in Glioma tissue.
ab32118 showing negative staining in Normal brain tissue.
Fluorescent immunohistochemical analysis of paraffin-embedded human normal kidney tissue using ab32118. Green-CK18 red-PI
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