Cytokeratin 18 antibody [E431-1] (ab32118)

Product Name 

Cytokeratin 18 antibody [E431-1]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [E431-1] to Cytokeratin 18

Immunogen 

A synthetic peptide corresponding to residues of human Cytokeratin 18.

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

Flow Cyt, ICC/IF, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/20.
ICC/IF: 1/100.
IHC-P: 1/100 - 1/250.

WB: 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

Is unsuitable for IP.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: A431 cell lysate.
IHC: Colon carcinoma.

Cellular localization 

Cytoplasmic

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated

Relevance 

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non keratinizing cells (ie. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.

Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

E431-1

Isotype 

IgG

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

See below for Cytokeratin 18 antibody [E431-1] images, references, products related to ab32118 and other tools.

Cytokeratin 18 antibody [E431-1] images:

References for Cytokeratin 18 antibody [E431-1] (ab32118)

  This product has been used in: (two most recent references)

Li X et al. Targeted overexpression of EZH2 in the mammary gland disrupts ductal morphogenesis and causes epithelial hyperplasia. Am J Pathol 175:1246-54 (2009). IHC-P; Mouse. PubMed: 19661437

Pratt MA et al. The canonical NF-kappaB pathway is required for formation of luminal mammary neoplasias and is activated in the mammary progenitor population. Oncogene 28:2710-22 (2009). WB; Mouse. PubMed: 19483731

See all 3 publication references for this product.


If you publish research using ab32118 please let us know so that we can cite the reference on this datasheet.

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