![Western blot - Cytokeratin 18 antibody [E431-1] (ab32118) image](#cytok18_wb.jpg)
![Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 18 antibody [E431-1] (ab32118) image](#citok18_ihc.jpg)
 image](#Cytokeratin-18-Primary-antibodies-ab32118-1.jpg)
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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Overview
Product name
Anti-Cytokeratin 18 antibody [E431-1]
See all Cytokeratin 18 products (35) ...
Description
Rabbit monoclonal [E431-1] to Cytokeratin 18
Tested applications
WB, IHC-P, ICC/IF, Flow Cytmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues of human Cytokeratin 18.
Positive control
WB: A431 cell lysate. IHC: Human colon carcinoma.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
E431-1
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/10000Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P: 1/100 - 1/250.
ICC/IF: 1/100
Flow Cyt: 1/20
Application notes
Is unsuitable for or IP.
Target
Function
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Tissue specificity
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
Involvement in disease
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
Sequence similarities
Belongs to the intermediate filament family.
Post-translational
modifications
Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
Cellular localization
Cytoplasm > perinuclear region.
Target information above from: UniProt accessionP05783
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- type I cytoskeletal 18 antibody
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
see all
Database links
- Entrez Gene: 3875 Human
- Omim: 148070 Human
- SwissProt: P05783 Human
- Unigene: 406013 Human
- Unigene: 598127 Human
Anti-Cytokeratin 18 antibody [E431-1] images:
Western blot - Cytokeratin 18 antibody [E431-1] (ab32118)
![Western blot - Cytokeratin 18 antibody [E431-1] (ab32118)](/ps/datasheet/Images/32/ab32118/cytok18_wb.jpg)
Anti-Cytokeratin 18 antibody [E431-1] (ab32118) at 1/10000 dilution + A431 cell lysate
Predicted band size : 48 kDa
Observed band size : 48 kDa
Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 18 antibody [E431-1] (ab32118)
![Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 18 antibody [E431-1] (ab32118)](/ps/datasheet/Images/32/ab32118/citok18_ihc.jpg)
Ab32118, at a dilution of 1/100, staining Cytokeratin 18 in paraffin embedded colon carcinoma tissue sections by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence-Cytokeratin 18 antibody [E431-1](ab32118)
](/ps/datasheet/images/32/ab32118/Cytokeratin-18-Primary-antibodies-ab32118-1.jpg)
ICC/IF image of ab32118 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32118, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Cytokeratin 18 antibody [E431-1] (ab32118)
This product has been referenced in:
- Lorenzatti Get al. CCN6 (WISP3) decreases ZEB1-mediated EMT and invasion by attenuation of IGF-1 receptor signaling in breast cancer. J Cell Sci 124:1752-8 (2011).Read more (PubMed: 21525039) »
- Gonzalez-Suarez Eet al. RANK ligand mediates progestin-induced mammary epithelial proliferation and carcinogenesis. Nature 468:103-7 (2010). IHC-P; Mouse.Read more (PubMed: 20881963) »
See all 5 publications for this product
Publishing research using ab32118? Please let us know so that we can cite the reference in this datasheet
![Cytokeratin 18 antibody [E431-1] for IHC-P in Mouse (32118)](/ps/datasheet/images/32/ab32118/Cytokeratin-18-Primary-antibodies-ab32118-2.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"