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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Overview
Product name
Anti-Cytokeratin 18 antibody
See all Cytokeratin 18 products (35) ...
Description
Rabbit polyclonal to Cytokeratin 18
Tested applications
WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
A synthetic peptide corresponding to residues on the C-terminus of human Cytokeratin 18
Positive control
A431 cell lysate, human urothelial carcinoma cells and HeLa cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 0.87% Sodium chloride, 0.09% Tris glycine
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/10,000 - 1/50,000.Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP
IP: 1/100IP: 1/100
Flow Cyt
Flow Cyt: 1/15Flow Cyt: 1/15
IHC-P: 1/250 - 1/500.(Antigen retrieval is recommended.)
ICC/IF: 1/250 - 1/500.
Target
Function
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Tissue specificity
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
Involvement in disease
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
Sequence similarities
Belongs to the intermediate filament family.
Post-translational
modifications
Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
Cellular localization
Cytoplasm > perinuclear region.
Target information above from: UniProt accessionP05783
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- type I cytoskeletal 18 antibody
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
see all
Database links
- Entrez Gene: 3875 Human
- Entrez Gene: 16668 Mouse
- Omim: 148070 Human
- SwissProt: P05783 Human
- SwissProt: P05784 Mouse
- Unigene: 406013 Human
- Unigene: 598127 Human
- Unigene: 22479 Mouse
Anti-Cytokeratin 18 antibody images:
Western blot - Cytokeratin 18 antibody (ab52948)
Anti-Cytokeratin 18 antibody (ab52948) at 1/50000 dilution + A431 cell lysate at 10 µg
Secondary
goat anti-rabbit HR at 1/2000 dilution
Predicted band size : 48 kDa
Observed band size : 48 kDa
Western blot - Cytokeratin 18 antibody (ab52948)
Anti-Cytokeratin 18 antibody (ab52948) at 1/250 dilution + Cytokeratin 18 protein (Human) (ab73638) at 0.01 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 1 minute
Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 18 antibody (ab52948)
ab52948 at 1/250 dilution staining Cytokeratin 18 in urothelial carcinoma cells by Immunohistochemistry, Paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - Cytokeratin 18 antibody (ab52948)
ab52948 at 1/250 dilution staining Cytokeratin 18 in HeLa cells by Immunofluorescence
References for Anti-Cytokeratin 18 antibody (ab52948)
This product has been referenced in:
- Krolewski RCet al. The generation of olfactory epithelial neurospheres in vitro predicts engraftment capacity following transplantation in vivo. Exp Neurol : (2011). ICC/IF; Mouse.Read more (PubMed: 21376038) »
- Sasaki Ret al. Oncogenic transformation of human ovarian surface epithelial cells with defined cellular oncogenes. Carcinogenesis 30:423-31 (2009).Read more (PubMed: 19126650) »
See all 3 publications for this product
Publishing research using ab52948? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"