Anti-Cytokeratin 18 (phospho S52) antibody (ab63393)

Overview

• Product nameAnti-Cytokeratin 18 (phospho S52) antibodySee all Cytokeratin 18 primary antibodies ...
• Description
  Rabbit polyclonal to Cytokeratin 18 (phospho S52)
• SpecificityThis antibody detects endogenous levels of Cytokeratin 18 only when phosphorylated at serine 52.
• Tested applicationsWB, ICC/IF, ELISA more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  A synthesized phosphopeptide derived from human Cytokeratin 18 around the phosphorylation site of serine 52 (STS^PFR)

• Positive controlHela cells and extracts from HepG2 cells

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg⁺² and Ca⁺²), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Intermediate Filaments
  • Class I
  • Cytokeratins

  • Tags & Cell Markers
  • Cell Type Markers
  • Tumor Associated

Applications

Target

• FunctionInvolved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
• Tissue specificityExpressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
• Involvement in diseaseDefects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
• Sequence similaritiesBelongs to the intermediate filament family.
• Post-translational
  modificationsPhosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
  Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
  O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
• Cellular localizationCytoplasm > perinuclear region.

Target information above from: UniProt accession P05783 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3875 Human
  • Omim: 148070 Human
  • SwissProt: P05783 Human
  • Unigene: 406013 Human

• Alternative names
  Cell proliferation inducing gene 46 protein antibodyCell proliferation inducing protein 46 antibodyCell proliferation-inducing gene 46 protein antibody

  CK 18 antibodyCK-18 antibodyCK18 antibodyCYK 18 antibodyCYK18 antibodyCytokeratin 18 antibodyCytokeratin endo B antibodyCytokeratin-18 antibodyK 18 antibodyK18 antibodyK1C18_HUMAN antibodyKeratin 18 antibodyKeratin D antibodykeratin, type I cytoskeletal 18 antibodyKeratin-18 antibodyKrt18 antibody

  see all

Anti-Cytokeratin 18 (phospho S52) antibody images

• 

  Immunocytochemistry/ Immunofluorescence - Cytokeratin 18 (phospho S52) antibody (ab63393)
  Immunofluorescence analysis of HeLa cells, using ab63393 at a 1:500 dilution.
  Left image untreated.
  Right image treated with phospho peptide.
• 

  Western blot - Cytokeratin 18 (phospho S52) antibody (ab63393)
  All lanes : Anti-Cytokeratin 18 (phospho S52) antibody (ab63393) at 1/500 dilution
  
  Lane 1 : Extracts from HepG2 cells
  Lane 2 : Extracts from HepG2 cells plus phospho peptide
  
  
  Predicted band size : 48 kDa
  Observed band size : 48 kDa