General notesab94060 is a 293T cell transfected lysate in which Human Cytokeratin 19 has been transiently over-expressed using a pCMV-Cytokeratin 19 plasmid. The lysate is provided in 1X Sample Buffer.
Note: For more detailed how the transfected lysate was prepared view preparation notes
BackgroundDevelopmental stage: Present in hair follicles at all stages of development.
Domain: This keratin differs from all other IF proteins in lacking the C-terminal tail domain.
Function: Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Similarity: Belongs to the intermediate filament family.
Tissue specificity: Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.