Anti-Cytokeratin 3/CK-3 antibody [AE5] (ab77869)
Key features and details
- Mouse monoclonal [AE5] to Cytokeratin 3/CK-3
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Rabbit, Cow, Human
- Isotype: IgG1
Overview
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Product name
Anti-Cytokeratin 3/CK-3 antibody [AE5]
See all Cytokeratin 3/CK-3 primary antibodies -
Description
Mouse monoclonal [AE5] to Cytokeratin 3/CK-3 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Rabbit, Cow, Human -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
AE5 -
Myeloma
P3-x63-Ag8 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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Flow Cyt |
Use 2µg for 106 cells.
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Notes |
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WB
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use 2µg for 106 cells. |
Target
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Tissue specificity
Cornea specific. -
Involvement in disease
Defects in KRT3 are a cause of Meesmann corneal dystrophy (MECD) [MIM:122100]; also abbreviated as MCD and known as juvenile epithelial corneal dystrophy of Meesmann. MECD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts. -
Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
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Database links
- Entrez Gene: 281267 Cow
- Entrez Gene: 3850 Human
- Omim: 148043 Human
- SwissProt: P12035 Human
- SwissProt: Q29426 Rabbit
- Unigene: 680652 Human
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Alternative names
- 65 kDa cytokeratin antibody
- CK-3 antibody
- CK3 antibody
see all
Images
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Overlay histogram showing A431 cells stained with ab77869 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77869, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab77869 has been referenced in 15 publications.
- Sun YC et al. Transmembrane Mucin 1 Blocks Fluorescein Ingress to Corneal Epithelium. Invest Ophthalmol Vis Sci 63:31 (2022). PubMed: 35212722
- Li M et al. Topical Vitamin C Promotes the Recovery of Corneal Alkali Burns in Mice. J Ophthalmol 2021:2406646 (2021). PubMed: 34976406
- Fernandes-Cunha GM et al. In situ-forming collagen hydrogel crosslinked via multi-functional PEG as a matrix therapy for corneal defects. Sci Rep 10:16671 (2020). PubMed: 33028837
- Zhang Y et al. The Role of the miR-21/SPRY2 Axis in Modulating Proangiogenic Factors, Epithelial Phenotypes, and Wound Healing in Corneal Epithelial Cells. Invest Ophthalmol Vis Sci 60:3854-3862 (2019). PubMed: 31529118
- Inamochi A et al. Simple oral mucosal epithelial transplantation in a rabbit model. Sci Rep 9:18088 (2019). PubMed: 31792300