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Anti-Cytokeratin 4 antibody [5H5] (ab117604)

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Overview

  • Product nameAnti-Cytokeratin 4 antibody [5H5]See all Cytokeratin 4 primary antibodies ...
  • Description
    Mouse monoclonal [5H5] to Cytokeratin 4
  • Tested applicationsSandwich ELISA, WB, IHC-P, ICC/IF more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human Cytokeratin 4 protein (CAA30534.1).

  • Positive controlHuman prostate tissue; A431 cells and cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab117604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA sELISA: Use at an assay dependent concentration.
WB WB: 1/500 - 1/1000. Predicted molecular weight: 57 kDa.
IHC-P IHC-P: Use a concentration of 5 µg/ml.
ICC/IF ICC/IF: Use a concentration of 10 µg/ml.

Target

  • Tissue specificityDetected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer.
  • Involvement in diseaseDefects in KRT4 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.
  • Sequence similaritiesBelongs to the intermediate filament family.

    • Target information above from: UniProt accession P19013 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • FormLocalisation: Intermediate filament (Cytoskeleton).
    • Alternative names
        CK 4 antibodyCK-4 antibodyCK4 antibody
        CYK4 antibodyCytokeratin 4 antibodyCytokeratin-4 antibodyCytokeratin4 antibodyFLJ31692 antibodyK2C4_HUMAN antibodyK4 antibodyKeratin 4 antibodyKeratin antibodyKeratin type II cytoskeletal 4 antibodyKeratin-4 antibodyKeratin4 antibodyKRT 4 antibodyKrt4 antibodytype II cytoskeletal 4 antibodyType-II keratin Kb4 antibody
      see all

    Anti-Cytokeratin 4 antibody [5H5] images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 4 antibody [5H5] (ab117604)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 4 antibody [5H5] (ab117604)
      ab117604, at 5µg/ml, staining Cytokeratin 4 in Formalin-Fixed, Paraffin-Embedded Human prostate tissue by immunohistochemistry.
    • Western blot - Anti-Cytokeratin 4 antibody [5H5] (ab117604)
      Western blot - Anti-Cytokeratin 4 antibody [5H5] (ab117604)
      Anti-Cytokeratin 4 antibody [5H5] (ab117604) + A431 cell lysate

      Predicted band size : 57 kDa
    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 4 antibody [5H5] (ab117604)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 4 antibody [5H5] (ab117604)
      ab117604, at 10µg/ml, staining Cytokeratin 4 in A431 cells by Immunofluorescence.
    • Sandwich ELISA - Anti-Cytokeratin 4 antibody [5H5] (ab117604)
      Sandwich ELISA - Anti-Cytokeratin 4 antibody [5H5] (ab117604)
      Detection limit for recombinant tagged Cytokeratin 4 is approximately 0.1ng/ml as a capture antibody.

    References for Anti-Cytokeratin 4 antibody [5H5] (ab117604)

    ab117604 has not yet been referenced specifically in any publications.

    Publishing research using ab117604 ? Please let us know so that we can cite the reference in this datasheet

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