Recombinant Anti-Cytokeratin 4 antibody [EPR1598] (ab92465)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1598] to Cytokeratin 4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cytokeratin 4 antibody [EPR1598]
See all Cytokeratin 4 primary antibodies -
Description
Rabbit monoclonal [EPR1598] to Cytokeratin 4 -
Host species
Rabbit -
Specificity
The immunogen shares high homology with other cytokeratins like cytokeratin-3(93%), cytokeratin-79(85.7%) and cytokeratin-2 oral(85.7%) etc. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human Cytokeratin 4.
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Positive control
- WB: A431 and Human uterus cell lysates IHC-P: Human tonsil tissue ICC/IF: A431 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1598 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | ||
IHC-P | ||
ICC/IF |
Notes |
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IHC-P: 1/100 - 1/250. Antigen retrieval is recommended before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
WB: 1/1000 - 1/10,000. Predicted molecular weight: 57 kDa.
Is unsuitable for Flow Cyt or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Tissue specificity
Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer. -
Involvement in disease
Defects in KRT4 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved. -
Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
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Database links
- Entrez Gene: 3851 Human
- Entrez Gene: 315323 Rat
- Omim: 123940 Human
- SwissProt: P19013 Human
- SwissProt: Q6IG00 Rat
- Unigene: 654610 Human
- Unigene: 706527 Human
- Unigene: 100688 Rat
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Form
Localisation: Intermediate filament (Cytoskeleton). -
Alternative names
- CK 4 antibody
- CK-4 antibody
- CK4 antibody
see all
Images
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ab92465, at a 1/100 dilution, staining Cytokeratin 4 in paraffin embedded Human tonsil tissue by Immunohistochemistry. Note positive staining of squamous cells.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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All lanes : Anti-Cytokeratin 4 antibody [EPR1598] (ab92465) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : Human uterus cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 57 kDa -
Immunofluorescent staining of A431 cells, using ab92465 at a 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab92465 has been referenced in 2 publications.
- Jerg A et al. Modulation of gingival cell response towards dental composites. Dent Mater 34:412-426 (2018). PubMed: 29306486
- Jacot TA et al. Development of a composite measure of product adherence, protocol compliance, and semen exposure using DNA and protein biomarkers for topical HIV prevention studies. PLoS One 9:e114368 (2014). ICC ; Human . PubMed: 25489736