![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 5 antibody [EPR1600Y] (ab75869) image](#ab75869ihc.gif)
![Western blot - Cytokeratin 5 antibody [EPR1600Y] (ab75869) image](#ab75869wb.gif)
 image](#Cytokeratin-5-Primary-antibodies-ab75869-1.jpg)
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Product name
Anti-Cytokeratin 5 antibody [EPR1600Y]
See all Cytokeratin 5 products (15) ...
Description
Rabbit monoclonal [EPR1600Y] to Cytokeratin 5
Tested applications
IHC-Fr, WB, IP, IHC-P, ICCmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in the head region of human Cytokeratin 5
Positive control
A431 cell lysate and human squamous cervical carcinoma tissue.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR1600Y
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 5 antibody [EPR1600Y] (ab75869)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 5 antibody [EPR1600Y] (ab75869) image](/ps/datasheet/Images/75/ab75869/ab75869ihc.gif)
(enlarge)
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Western blot - Cytokeratin 5 antibody [EPR1600Y] (ab75869)
![Western blot - Cytokeratin 5 antibody [EPR1600Y] (ab75869) image](/ps/datasheet/Images/75/ab75869/ab75869wb.gif)
(enlarge)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 5 antibody [EPR1600Y](ab75869)
 image](/ps/datasheet/images/75/ab75869/Cytokeratin-5-Primary-antibodies-ab75869-1.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC: 1/250 - 1/500.
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: Use at an assay dependent dilution (PMID 19949112).
IP: 1/60.
WB: 1/10,000 - 1/20,000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
Is unsuitable for Flow Cyt.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Involvement in disease
Defects in KRT5 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
Defects in KRT5 are the cause of epidermolysis bullosa simplex with migratory circinate erythema (EBSMCE) [MIM:609352]. EBSMCE is a form of intraepidermal epidermolysis bullosa characterized by unusual migratory circinate erythema. Skin lesions appear from birth primarily on the hands, feet, and legs but spare nails, ocular epithelia and mucosae. Lesions heal with brown pigmentation but no scarring. Electron microscopy findings are distinct from those seen in the DM-EBS, with no evidence of tonofilament clumping.
Defects in KRT5 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.
Defects in KRT5 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, althought it is less severe.
Defects in KRT5 are the cause of epidermolysis bullosa simplex with mottled pigmentation (MP-EBS) [MIM:131960]. MP-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering at acral sites and 'mottled' pigmentation of the trunk and proximal extremities with hyper- and hypopigmentation macules.
Defects in KRT5 are the cause of Dowling-Degos disease (DDD) [MIM:179850]; also known as Dowling-Degos-Kitamura disease or reticulate acropigmentation of Kitamura. DDD is an autosomal dominant genodermatosis. Affected individuals develop a postpubertal reticulate hyperpigmentation that is progressive and disfiguring, and small hyperkeratotic dark brown papules that affect mainly the flexures and great skin folds. Patients usually show no abnormalities of the hair or nails.
Sequence similarities
Belongs to the intermediate filament family.
Target information above from: UniProt accessionP13647
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- type II cytoskeletal 5 antibody
- 58 kDa cytokeratin antibody
- CK 5 antibody
see all
Database links
- Entrez Gene: 3852 Human
- Omim: 148040 Human
- SwissProt: P13647 Human
- Unigene: 433845 Human
Anti-Cytokeratin 5 antibody [EPR1600Y] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 5 antibody [EPR1600Y] (ab75869)
ab75869 at 1/100 dilution staining Cytokeratin 5 in human squamous cervical carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Western blot - Cytokeratin 5 antibody [EPR1600Y] (ab75869)
Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869) at 1/10000 dilution + A431 cell lysate at 10 µg
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 62 kDa
Observed band size : 62 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 5 antibody [EPR1600Y](ab75869)
Fluorescent immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue using ab75869. Green-CK5 red-PI
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 5 antibody [EPR1600Y](ab75869)
](/ps/datasheet/images/75/ab75869/Cytokeratin-5-Primary-antibodies-ab75869-2.jpg)
Fluorescent immunohistochemical analysis of paraffin-embedded human cervical carcinoma using ab75869. Green-CK5 red-PI.
References for Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869)
This product has been referenced in:
- Nobrega C et al. Dissemination of mycobacteria to the thymus renders newly generated T cells tolerant to the invading pathogen. J Immunol 184:351-8 (2010). IHC-Fr; Mouse.Read more (PubMed: 19949112) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"