Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab17130? Please let us know so that we can cite the reference in this datasheet
ab17130 has been referenced in 1 publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
ab17130 staining Cytokeratin 5 in Human keratinocytes by ICC (Immunocytochemistry). Cells were fixed with ethanol:acetone (1:1), permeabilized with Triton X 100 (0.1% for 5 minutes) and blocked with FCS/H2O2 3% (endo. PO) for 5 minutes at 21ºC. Samples were incubated with primary antibody (1/100 in FBS Stain-Buffer) for 1 hour at 21ºC. An undiluted HRP-conjugated Goat anti-rabbit/mouse IgG polyclonal was used as the secondary antibody. Negative cells were stained with Mayer's hematoxilin.
This image is courtesy of an anonymous Abreview
3
Call 01223 696 000 or contact us