![Immunocytochemistry - Cytokeratin 5 antibody [XM26] (ab17130) image](#Cytokeratin-5-Primary-antibodies-ab17130-4.jpg)
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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Overview
Product name
Anti-Cytokeratin 5 antibody [XM26]
See all Cytokeratin 5 products (13) ...
Description
Mouse monoclonal [XM26] to Cytokeratin 5
Tested applications
IHC-P, IHC-Fr, ICCmore details
Cross reactivity
Reacts with
Human
Immunogen
Prokaryotic recombinant protein corresponding to 103 amino acid portion of the C-terminal region of the cytokeratin 5 molecule (Human).
Positive control
Skin
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, Tissue culture supernatant
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
XM26
Isotype
IgG1
Light chain type
kappa
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
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Immunocytochemistry - Cytokeratin 5 antibody [XM26] (ab17130)
![Immunocytochemistry - Cytokeratin 5 antibody [XM26] (ab17130) image](/ps/datasheet/images/17/ab17130/Cytokeratin-5-Primary-antibodies-ab17130-4.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab17130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
IHC-Fr
Three stars (1 Abreview)
Application notes
IHC-P: 1/50 - 1/75. An incubation period of 30 minutes at room temperature is recommended. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
IHC-Fr: Use at an assay dependent dilution (see PMID: 17065488).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Involvement in disease
Defects in KRT5 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
Defects in KRT5 are the cause of epidermolysis bullosa simplex with migratory circinate erythema (EBSMCE) [MIM:609352]. EBSMCE is a form of intraepidermal epidermolysis bullosa characterized by unusual migratory circinate erythema. Skin lesions appear from birth primarily on the hands, feet, and legs but spare nails, ocular epithelia and mucosae. Lesions heal with brown pigmentation but no scarring. Electron microscopy findings are distinct from those seen in the DM-EBS, with no evidence of tonofilament clumping.
Defects in KRT5 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.
Defects in KRT5 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, althought it is less severe.
Defects in KRT5 are the cause of epidermolysis bullosa simplex with mottled pigmentation (MP-EBS) [MIM:131960]. MP-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering at acral sites and 'mottled' pigmentation of the trunk and proximal extremities with hyper- and hypopigmentation macules.
Defects in KRT5 are the cause of Dowling-Degos disease (DDD) [MIM:179850]; also known as Dowling-Degos-Kitamura disease or reticulate acropigmentation of Kitamura. DDD is an autosomal dominant genodermatosis. Affected individuals develop a postpubertal reticulate hyperpigmentation that is progressive and disfiguring, and small hyperkeratotic dark brown papules that affect mainly the flexures and great skin folds. Patients usually show no abnormalities of the hair or nails.
Sequence similarities
Belongs to the intermediate filament family.
Target information above from: UniProt accessionP13647
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- type II cytoskeletal 5 antibody
- 58 kDa cytokeratin antibody
- CK 5 antibody
see all
Database links
- Entrez Gene: 3852 Human
- Omim: 148040 Human
- SwissProt: P13647 Human
- Unigene: 433845 Human
Anti-Cytokeratin 5 antibody [XM26] images:
Immunocytochemistry - Cytokeratin 5 antibody [XM26] (ab17130)
ab17130 staining Cytokeratin 5 in Human keratinocytes by ICC (Immunocytochemistry). Cells were fixed with ethanol:acetone (1:1), permeabilized with Triton X 100 (0.1% for 5 minutes) and blocked with FCS/H2O2 3% (endo. PO) for 5 minutes at 21°C. Samples were incubated with primary antibody (1/100 in FBS Stain-Buffer) for 1 hour at 21°C. An undiluted HRP-conjugated Goat anti-rabbit/mouse IgG polyclonal was used as the secondary antibody. Negative cells were stained with Mayer's hematoxilin.
This image is courtesy of an anonymous Abreview
References for Anti-Cytokeratin 5 antibody [XM26] (ab17130)
This product has been referenced in:
- Yoshida Set al. Cytokeratin 15 can be used to identify the limbal phenotype in normal and diseased ocular surfaces. Invest Ophthalmol Vis Sci 47:4780-6 (2006).Read more (PubMed: 17065488) »
See 1 publication for this product
Publishing research using ab17130? Please let us know so that we can cite the reference in this datasheet
![Cytokeratin 5 antibody [XM26] for Immunocytochemistry in Human (17130)](/ps/datasheet/images/17/ab17130/Cytokeratin-5-Primary-antibodies-ab17130-3.jpg)
![Cytokeratin 5 antibody [XM26] for IHC-Fr in Human (17130)](/ps/datasheet/images/17/ab17130/Cytokeratin-5-Primary-antibodies-ab17130-1.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"