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Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 5 antibody (ab24647)
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Immunohistochemistry - Cytokeratin 5 antibody (ab24647)
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Immunocytochemistry/ Immunofluorescence-Cytokeratin 5 antibody(ab24647)
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Product Name
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Cytokeratin 5 antibody
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See all Cytokeratin 5 antibodies (11)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Cytokeratin 5
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Immunogen
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Synthetic peptide sequence derived from the C-terminus of the mouse keratin 5 protein, KFVSTTSSSRRSPKS.
BLAST 'KFVSTTSSSR RSPKS' with  or 
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Rat
(Due to sequence homology)
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Specificity
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Recognises Keratin 5.
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Tested applications
(see key)
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ICC/IF, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/1000.
IHC-P: 1/1000.
IHC-Fr: Use at an assay dependent dilution (PMID 18414681).
WB: 1/1000. Predicted molecular weight: 60 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Epidermal tissue such as the esophagus.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
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Relevance
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Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinising tissue (ie., skin) and non keratinising cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of 40 to 70 kDa and isoelectric points of 5 to 8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Defects in KRT5 are a cause of epidermolysis bullosa simplex.
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Cytokeratin 5 antibody (ab24647) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.03% Thimerosal (merthiolate)
Constituents: PBS Material safety datasheet (MSDS) for this product:
Thimerosal MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cytokeratin 5 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Cytokeratin 5 antibody images, references, products related to ab24647 and other tools.
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Cytokeratin 5 antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 5 antibody (ab24647)
Immunofluorescence staining of epithelial layers from esophagus of an 18 day day old mouse embryo showing staining of nucleus and the epithelial layer
Y.Hu, UCSD
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Immunohistochemistry - Cytokeratin 5 antibody (ab24647)
Visualisation of nuclei (purple) and nondifferentiated cells (red) reveal the marked expansion of non-differentiated cells in IKK alpha mutant epidermis
Y. Hu, UCSD
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Immunocytochemistry/ Immunofluorescence-Cytokeratin 5 antibody(ab24647)
ICC/IF image of ab24647 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24647, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for Cytokeratin 5 antibody (ab24647)
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This product has been used in: (two most recent references)
Parker J et al. A 3-D well-differentiated model of pediatric bronchial epithelium demonstrates unstimulated morphological differences between asthmatic and nonasthmatic cells. Pediatr Res 67:17-22 (2010). ICC/IF; Human. PubMed: 19755931
Wang W et al. Increased p53 immunoreactivity in proliferative inflammatory atrophy of prostate is related to focal acute inflammation. APMIS 117:185-95 (2009). IHC-P; Human. PubMed: 19245591
See all 11 publication references for this product.
If you publish research using ab24647 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Cytokeratin 5
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Cytokeratin 5 antibody - more information
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Cytokeratin 5 antibody - related products:
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