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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Overview
Product name
Anti-Cytokeratin 5 antibody
See all Cytokeratin 5 products (13) ...
Description
Rabbit polyclonal to Cytokeratin 5
Specificity
ab53121 detects endogenous levels of total Cytokeratin 5 protein.
Tested applications
ELISA, IHC-P, ICC/IF, IHC-FoFr, WBmore details
Cross reactivity
Reacts with
Mouse, Human, Pig
Immunogen
A synthetic peptide derived from human Cytokeratin 5.
Positive control
HepG2 cell extracts and human breast carcinoma tissue.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab53121 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/10000ELISA: 1/10000
IHC-P: 1/50 - 1/100.
ICC/IF: Use a concentration of 1 µg/ml
IHC-FoFr: Use at an assay dependent dilution. (PubMed: 21278345)
WB: 1/300 - 1/1000.Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
Target
Involvement in disease
Defects in KRT5 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
Defects in KRT5 are the cause of epidermolysis bullosa simplex with migratory circinate erythema (EBSMCE) [MIM:609352]. EBSMCE is a form of intraepidermal epidermolysis bullosa characterized by unusual migratory circinate erythema. Skin lesions appear from birth primarily on the hands, feet, and legs but spare nails, ocular epithelia and mucosae. Lesions heal with brown pigmentation but no scarring. Electron microscopy findings are distinct from those seen in the DM-EBS, with no evidence of tonofilament clumping.
Defects in KRT5 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.
Defects in KRT5 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, althought it is less severe.
Defects in KRT5 are the cause of epidermolysis bullosa simplex with mottled pigmentation (MP-EBS) [MIM:131960]. MP-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering at acral sites and 'mottled' pigmentation of the trunk and proximal extremities with hyper- and hypopigmentation macules.
Defects in KRT5 are the cause of Dowling-Degos disease (DDD) [MIM:179850]; also known as Dowling-Degos-Kitamura disease or reticulate acropigmentation of Kitamura. DDD is an autosomal dominant genodermatosis. Affected individuals develop a postpubertal reticulate hyperpigmentation that is progressive and disfiguring, and small hyperkeratotic dark brown papules that affect mainly the flexures and great skin folds. Patients usually show no abnormalities of the hair or nails.
Sequence similarities
Belongs to the intermediate filament family.
Target information above from: UniProt accessionP13647
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- type II cytoskeletal 5 antibody
- 58 kDa cytokeratin antibody
- CK 5 antibody
see all
Database links
- Entrez Gene: 3852 Human
- Entrez Gene: 110308 Mouse
- Omim: 148040 Human
- SwissProt: P13647 Human
- SwissProt: Q922U2 Mouse
- Unigene: 433845 Human
- Unigene: 451847 Mouse
Anti-Cytokeratin 5 antibody images:
Western blot - Cytokeratin 5 antibody (ab53121)
All lanes : Anti-Cytokeratin 5 antibody (ab53121) at 1/300 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HepG2 cell extract with immunising peptide
Predicted band size : 62 kDa
Observed band size : 62 kDa
Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 5 antibody (ab53121)
ab53121 at 1/50 dilution staining Cytokeratin 5 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
Immunocytochemistry/ Immunofluorescence-Cytokeratin 5 antibody(ab53121)
ICC/IF image of ab53121 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53121, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Cytokeratin 5 antibody (ab53121)
This product has been referenced in:
- Sanos SLet al. Stromal cell networks regulate thymocyte migration and dendritic cell behavior in the thymus. J Immunol 186:2835-41 (2011). IHC-FoFr; Mouse.Read more (PubMed: 21278345) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"