Recombinant
RabMAb

Anti-Cytokeratin 6 antibody [EPR1603Y] (ab52620)

Overview

  • Product nameAnti-Cytokeratin 6 antibody [EPR1603Y]
    See all Cytokeratin 6 primary antibodies
  • Description
    Rabbit monoclonal [EPR1603Y] to Cytokeratin 6
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Cytokeratin 6 aa 550-650 (C terminal). P48668, P02538 and P04259.
    Database link: P48668

  • Positive control
    • A431 cell lysate, human squamous cervical carcinoma, A431 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab52620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100000. Predicted molecular weight: 60 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • Tissue specificityConstitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.
    • Sequence similaritiesBelongs to the intermediate filament family.
    • Information by UniProt
    • Database links
    • Alternative names
      • CK 6A antibody
      • CK 6B antibody
      • CK 6C antibody
      • CK 6D antibody
      • CK 6E antibody
      • CK-6C antibody
      • CK-6E antibody
      • Cytokeratin 6a antibody
      • Cytokeratin 6B antibody
      • Cytokeratin 6C antibody
      • Cytokeratin 6D antibody
      • Cytokeratin 6E antibody
      • Cytokeratin-6C antibody
      • Cytokeratin-6E antibody
      • K2C6C_HUMAN antibody
      • K6a keratin antibody
      • K6b keratin antibody
      • K6C antibody
      • K6c keratin antibody
      • K6d keratin antibody
      • K6e keratin antibody
      • Keratin antibody
      • Keratin K6h antibody
      • Keratin type II cytoskeletal 6A antibody
      • Keratin type II cytoskeletal 6B antibody
      • Keratin type II cytoskeletal 6C antibody
      • Keratin type II cytoskeletal 6D antibody
      • Keratin type II cytoskeletal 6E antibody
      • Keratin-6C antibody
      • KRT6A antibody
      • KRT6B antibody
      • KRT6C antibody
      • KRT6D antibody
      • KRT6E antibody
      • type II cytoskeletal 6C antibody
      • Type-II keratin Kb12 antibody
      see all

    Anti-Cytokeratin 6 antibody [EPR1603Y] images

    • Immunocytochemistry/Immunofluorescence analysis of HaCaT (Human keratinocyte cell line) labelling Cytokeratin 6 with purified ab52620 at 1/1000. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    • Anti-Cytokeratin 6 antibody [EPR1603Y] (ab52620) at 1/100000 dilution + A431 cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP antibody at 1/2000 dilution

      Predicted band size : 60 kDa
      Observed band size : 60 kDa
    • Ab52620 at 1/100-1/250 dilution staining human squamous cervical carcinoma; paraffin embedded.
    • Ab52620 at 1/100-1/250 dilution staining A431 cells.

    References for Anti-Cytokeratin 6 antibody [EPR1603Y] (ab52620)

    This product has been referenced in:

    See 1 Publication for this product

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    Thank you for contacting us.

    The exact ab52620’s immunogen shares 40% identity with Cytokeratin 14 (UniProt: P02533). I would not predict cross-reactivity, but it is not guaranteed without testing.

    I hope this helps and if I c...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"