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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Anti-Cytokeratin 8 + 18 + 19 antibody [2A4] (ab41825)
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Related products
- Secondary antibodies
- Isotype controls
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab41825? Please let us know so that we can cite the reference in this datasheet
ab41825 has been referenced in 1 publications.
- Kelley R et al. Tubular cell-enriched subpopulation of primary renal cells improves survival and augments kidney function in rodent model of chronic kidney disease. Am J Physiol Renal Physiol 299:F1026-39 (2010). Flow Cyt; Rat. PubMed: 20826573
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
](/ps/datasheet/images/41/ab41825/Cytokeratin-8-18-19-Primary-antibodies-ab41825-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Cytokeratin 8 + 18 + 19 antibody [2A4](ab41825)
IHC image of ab41825 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41825, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
](/ps/datasheet/images/41/ab41825/Cytokeratin-8-18-19-Primary-antibodies-ab41825-2.jpg)
Flow Cytometry-Anti-Cytokeratin 8 + 18 + 19 antibody [2A4](ab41825)
Overlay histogram showing MCF7 cells stained with ab41825 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41825, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
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