Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab41825 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
|
|||||||||
|
|||||||||
Inquiry: Dear Sir/ Madam, One customer raised an inquiry about ab118855(Anti-Keratin 8+18+19 antibody [2A4]) and ab41825 (Anti-Cytokeratin 8 + 18 + 19 antibody [2A4]). She found that these 2 antibodies are both for Cytokeratin 8 + 18 + 19 and raised from the same clone: 2A4. Therefore, she would like to ask whether these 2 antibodies are the same antibody. Beside, which antibody is recommended for ICC/IF? Would you please help her resolve the problems? Thank you very much. Best regards, |
|||||||||
ANSWER: |
Thank you for your enquiry regarding ab41825 and ab118855. |
||||||||
|
|||||||||
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
IHC image of ab41825 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41825, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Overlay histogram showing MCF7 cells stained with ab41825 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41825, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
0
Call 01223 696 000 or contact us