Cytokeratin 8 + 18 + 19 antibody [2A4] (ab41825)

Product Name 

Cytokeratin 8 + 18 + 19 antibody [2A4]

Product type 

Primary antibodies

Description 

Mouse monoclonal [2A4] to Cytokeratin 8 + 18 + 19

Immunogen 

Cytokeratins isolated from cultured pig kidney epithelial cells.

Reacts with 
(species key)

Hu, Pig

Tested applications 
(see key)

IHC-Fr, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
IHC-Fr: Use at an assay dependent dilution.
WB: 1/1000.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins

Relevance 

The cytokeratins are intermediate filament proteins responsible for the structural integrity of epithelial cells.
Keratin proteins belong to 2 families: acidic (or type I) and basic (or type II). The acidic keratins are coded by genes KRT9 to 19, the basic keratins by genes KRT1 to 8. Cytokeratins 8, 18 and 19 are encoded by the genes KRT8, 18 and 19 respectively.
Type I and II keratins are usually coordinately synthesized in preferential pairs so that at least 1 member of each family is expressed in each epithelial cell, in equal proportions.
Cytokeratin 8, together with its filament partner cytokeratin 18, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues. KRT8/18 mutations may predispose to liver disease, including cryptogenic cirrhosis, in humans.
Unlike its related family members, cytokeratin 19 (the smallest known acidic cytokeratin at 40kDa) is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. It is often expressed in cultured epithelial cells and in some carcinomas.

This antibody recognizes cytokeratins 8, 18 and 19 typical for simple epithelial cells.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

2A4

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C.

Notes 

Balb/c spleen cells used to produce hybridoma.

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