You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-Cytokeratin 8 + 18 + 19 antibody [2A4] (ab41825)

CodeSizePriceAbpointsAvailability
    
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-Cytokeratin 8 + 18 + 19 antibody [2A4]

Description

Mouse monoclonal [2A4] to Cytokeratin 8 + 18 + 19

Tested applications

WB, IHC-P, IHC-Fr, Flow Cytmore details

Cross reactivity

Reacts with

Rat, Human, Pig

Immunogen

Cytokeratins isolated from cultured pig kidney epithelial cells.

General notes

Balb/c spleen cells used to produce hybridoma.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Primary antibody notes

This antibody recognizes cytokeratins 8, 18 and 19 typical for simple epithelial cells.

Clonality

Monoclonal

Clone number

2A4

Isotype

IgG1

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Cytokeratin 8 + 18 + 19 antibody [2A4](ab41825)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Cytokeratin 8 + 18 + 19 antibody [2A4](ab41825) image (enlarge)

  • Flow Cytometry-Anti-Cytokeratin 8 + 18 + 19 antibody [2A4](ab41825)Flow Cytometry-Anti-Cytokeratin 8 + 18 + 19 antibody [2A4](ab41825) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab41825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • WB

     WB: 1/1000

    WB: 1/1000

  • ShowHide1 Image

    IHC-P

     IHC-P: Use a concentrati...Read more →

    IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • ShowHide

    IHC-Fr

     IHC-Fr: Use at an assay ...Read more →

    IHC-Fr: Use at an assay dependent dilution.

  • ShowHide1 Image

    Flow Cyt

      See more...Read more →

    Flow Cyt: Use 1µg for 106 cells.

Target

Relevance

The cytokeratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. Keratin proteins belong to 2 families: acidic (or type I) and basic (or type II). The acidic keratins are coded by genes KRT9 to 19, the basic keratins by genes KRT1 to 8. Cytokeratins 8, 18 and 19 are encoded by the genes KRT8, 18 and 19 respectively. Type I and II keratins are usually coordinately synthesized in preferential pairs so that at least 1 member of each family is expressed in each epithelial cell, in equal proportions. Cytokeratin 8, together with its filament partner cytokeratin 18, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues. KRT8/18 mutations may predispose to liver disease, including cryptogenic cirrhosis, in humans. Unlike its related family members, cytokeratin 19 (the smallest known acidic cytokeratin at 40kDa) is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. It is often expressed in cultured epithelial cells and in some carcinomas.

Cellular localization

Cytoplasmic

Alternative names

  • CARD2 antibody
  • CARD2 antibody
  • CK18 antibody
  • CK19 antibody
  • CK8 antibody
  • CYK18 antibody
  • K18 antibody
  • K19 antibody
  • K1CS antibody
  • K2C8 antibody
  • K8 antibody
  • keratin 18 antibody
  • keratin 19 antibody
  • keratin 8 antibody
  • Keratin type I 40kDa antibody
  • Keratin type I cytoskeletal 18 antibody
  • Keratin type I cytoskeletal 19 antibody
  • Keratin type II cytoskeletal 8 antibody
  • KO antibody
  • KRT18 antibody
  • KRT19 antibody
  • KRT8 antibody
  • MGC15366 antibody
see all

Anti-Cytokeratin 8 + 18 + 19 antibody [2A4] images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Cytokeratin 8 + 18 + 19 antibody [2A4](ab41825)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Cytokeratin 8 + 18 + 19 antibody [2A4](ab41825)

IHC image of ab41825 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41825, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  Flow Cytometry-Anti-Cytokeratin 8 + 18 + 19 antibody [2A4](ab41825)

Flow Cytometry-Anti-Cytokeratin 8 + 18 + 19 antibody [2A4](ab41825)

Overlay histogram showing MCF7 cells stained with ab41825 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41825, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-Cytokeratin 8 + 18 + 19 antibody [2A4] (ab41825)

This product has been referenced in:

  • Kelley Ret al. Tubular cell-enriched subpopulation of primary renal cells improves survival and augments kidney function in rodent model of chronic kidney disease. Am J Physiol Renal Physiol 299:F1026-39 (2010). Flow Cyt; Rat.Read more (PubMed: 20826573) »

See 1 publication for this product

Publishing research using ab41825? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"