|
|
|
|
Western blot - Cytokeratin 8 antibody [E432] (ab32357)
![Western blot - Cytokeratin 8 antibody [E432] (ab32357)](/ps/datasheet/images/Wb cytok 8.jpg)
(enlarge)
|
|
Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 8 antibody [E432] (ab32357)
![Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 8 antibody [E432] (ab32357)](/ps/datasheet/images/IHC cytok 8.jpg)
(enlarge)
|
|
|
|
|
Product Name
|
Cytokeratin 8 antibody [E432]
|
| |
See all Cytokeratin 8 antibodies (29)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit monoclonal [E432] to Cytokeratin 8
|
|
Immunogen
|
Synthetic peptide corresponding to residues surrounding Ser73 of human Cytokeratin 8.
|
|
Reacts with
|
Hu
|
|
Does not react with
(species key)
|
Ms, Rat
|
|
Specificity
|
Ab32357 recognises cytokeratin 8.
|
|
Tested applications
(see key)
|
Flow Cyt, ICC, IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
Flow Cyt: 1/20.
ICC: 1/50.
IHC-P: 1/250 - 1/500.
WB: 1/500. Predicted molecular weight: 54 kDa.
Is unsuitable for IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
WB: A431 cell lysate.
IHC-P: stomach carcinoma.
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Other
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
|
|
Relevance
|
Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
E432
|
|
Isotype
|
IgG
|
|
Storage buffer
|
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 50mM Tris glycine, 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
|
|
Notes
|
Produced under U.S. Patent No. 5,675,063.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cytokeratin 8 antibody [E432] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for Cytokeratin 8 antibody [E432] images, references, products related to ab32357 and other tools.
|
| |
Cytokeratin 8 antibody [E432] images:
|
|
|
| |
 Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 8 antibody [E432] (ab32357)
Ab32357, at a dilution of 1/250, staining Cytokeratin 8 in paraffin embedded stomach carcinoma tissue by Immunohistochemistry.
|
| |
| |
|
Search PubMed (MEDLINE) for references to Cytokeratin 8
|
| |
Cytokeratin 8 antibody [E432] - more information
|
Customer reviews (feedback) regarding Cytokeratin 8 antibody [E432] |
Customer FAQs regarding Cytokeratin 8 antibody [E432] |
Protocols for Cytokeratin 8 antibody [E432] |
Price and availability of products related to Cytokeratin 8 antibody [E432] |
| |
Cytokeratin 8 antibody [E432] - related products:
|
|
|
| |
Cytokeratin 8 antibody [E432] - other tools:
|
Contact Abcam with a Technical Enquiry about Cytokeratin 8 antibody [E432] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - Cytokeratin 8 antibody [E432] (ab32357)](/index.html?datasheet=32357#Wb cytok 8.jpg)
![Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 8 antibody [E432] (ab32357)](/index.html?datasheet=32357#IHC cytok 8.jpg)
