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Anti-Cytokeratin 8 antibody [E432] (ab32357)

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Overview

Product name

Anti-Cytokeratin 8 antibody [E432]
See all Cytokeratin 8 products (34) ...

Description

Rabbit monoclonal [E432] to Cytokeratin 8

Specificity

Ab32357 recognises cytokeratin 8.

Tested applications

WB, ICC, IHC-P, Flow Cytmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

Synthetic peptide corresponding to residues surrounding Ser73 of human Cytokeratin 8.

Positive control

WB: A431 cell lysate. IHC-P: stomach carcinoma.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 50mM Tris glycine, 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Clonality

Monoclonal

Clone number

E432

Isotype

IgG

Research areas

Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Other
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins

  • Western blot - Cytokeratin 8 antibody [E432] (ab32357)Western blot - Cytokeratin 8 antibody [E432] (ab32357) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 8 antibody [E432] (ab32357)Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 8 antibody [E432] (ab32357) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500Predicted molec...Read more →

    WB: 1/500Predicted molecular weight: 54 kDa.

  • ICC

     ICC: 1/50

    ICC: 1/50

  • 1 Image

    IHC-P

     IHC-P: 1/250 - 1/500.

    IHC-P: 1/250 - 1/500.

  • Flow Cyt

     Flow Cyt: 1/20

    Flow Cyt: 1/20

Application notes

Is unsuitable for or IP.

Target

Function

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Tissue specificity

Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.

Involvement in disease

Defects in KRT8 are a cause of cirrhosis (CIRRH) [MIM:215600].

Sequence similarities

Belongs to the intermediate filament family.

Post-translational
modifications

Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.

Cellular localization

Cytoplasm. Nucleus > nucleoplasm. Nucleus matrix.

Target information above from: UniProt accessionP05787 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • card2 antibody
  • Cardiac autoantigen 2 120kD antibody
  • CK 8 antibody
  • CK-8 antibody
  • ck8 antibody
  • Cyk 8 antibody
  • cyk8 antibody
  • CYKER antibody
  • Cytokeratin 8 antibody
  • Cytokeratin endo A antibody
  • Cytokeratin-8 antibody
  • Cytokeratin8 antibody
  • DreK8 antibody
  • EndoA antibody
  • k0 antibody
  • k2c8 antibody
  • K2C8_HUMAN antibody
  • k8 antibody
  • Keratin 8 antibody
  • Keratin type ii cytoskeletal 8 antibody
  • Keratin, type II cytoskeletal 8 antibody
  • Keratin-8 antibody
  • Keratin8 antibody
  • KO antibody
  • Krt 2.8 antibody
  • Krt 8 antibody
  • krt8 antibody
  • MGC118110 antibody
  • MGC174782 antibody
  • MGC53564 antibody
  • MGC85764 antibody
  • sb:cb186 antibody
  • Type-II keratin Kb8 antibody
see all

Database links

Anti-Cytokeratin 8 antibody [E432] images:

  Western blot - Cytokeratin 8 antibody [E432] (ab32357)

Western blot - Cytokeratin 8 antibody [E432] (ab32357)

Anti-Cytokeratin 8 antibody [E432] (ab32357) at 1/500 dilution + A431 cell lysate

Predicted band size : 54 kDa
Observed band size : 57 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 8 antibody [E432] (ab32357)

Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 8 antibody [E432] (ab32357)

Ab32357, at a dilution of 1/250, staining Cytokeratin 8 in paraffin embedded stomach carcinoma tissue by Immunohistochemistry.

Protocols

  • Recommended IHC-P protocol with ab32357

    • References for Anti-Cytokeratin 8 antibody [E432] (ab32357)

    ab32357 has not yet been referenced specifically in any publications.

    Publishing research using ab32357? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"