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Western blot - Cytokeratin 8 antibody [M20] (ab9023)
![Western blot - Cytokeratin 8 antibody [M20] (ab9023)](/ps/datasheet/images/ab9023_1.jpg)
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Immunocytochemistry/ Immunofluorescence - Cytokeratin 8 antibody [M20] (ab9023)
![Immunocytochemistry/ Immunofluorescence - Cytokeratin 8 antibody [M20] (ab9023)](/ps/datasheet/images/ab9023_2.jpg)
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Immunocytochemistry/ Immunofluorescence - Cytokeratin 8 antibody [M20] (ab9023)
![Immunocytochemistry/ Immunofluorescence - Cytokeratin 8 antibody [M20] (ab9023)](/ps/datasheet/images/ab9023_3.jpg)
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Product Name
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Cytokeratin 8 antibody [M20]
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See all Cytokeratin 8 antibodies (29)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [M20] to Cytokeratin 8
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Immunogen
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Keratin isolated from the human breast carcinoma cell line MCF-7.
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Reacts with
(species key)
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Hu, Ms
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Specificity
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Reacts with glandular epithelial cells (endocrine and exocrine) as well as mesothelial cells of the digestive, respiratory and urogenital tract and most adenocarcinomas derived from these cells.
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
ICC/IF: 1/100.
IHC-P: Use at a concentration of 4 µg/ml.
IHC-Fr: 1/100 - 1/200.
WB: 1/100 - 1/1000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Other
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
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Relevance
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Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides.
They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8.
The individual cytokeratin polypeptides are numbered 1 to 20.
The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium.
Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.
The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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M20
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cytokeratin 8 antibody [M20] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Cytokeratin 8 antibody [M20] images, references, products related to ab9023 and other tools.
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Cytokeratin 8 antibody [M20] images:
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 Western blot - Cytokeratin 8 antibody [M20] (ab9023)
The image shows different concentration of total protein from normal and tumour colon tissues. This picture was kindly supplied as part of the review submitted by Semona Rupchand.
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Immunocytochemistry/ Immunofluorescence - Cytokeratin 8 antibody [M20] (ab9023)
ab9023 staining MCF-7 breast cancer cells, grown in 8 well chamber slides, by ICC/IF. Cells were fixed with Acetone for 20 minutes at -20°C and permabilized with 0.1% Triton X-100 for 5 minutes at 4°C prior to blocking in 1% fish gelatin for 1 hour at RT. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour. An Alexa Fluor® 488 conjugated donkey anti-mouse antibody diluted 1/1000 was used as the secondary. Slides were mounted using mounting medium containing DAPI. This image is courtesy of an anonymous Abreview
See Abreview
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Immunocytochemistry/ Immunofluorescence - Cytokeratin 8 antibody [M20] (ab9023)
Ab9023 staining cells from a human pancreatic cancer cell line by ICC/IF. The cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C. Ab9023 was diluted 1/100 and incubated with the sample for 1 hour at 22°C. An Alexa Fluor® 488 conjugated donkey anti-mouse antibody, diluted 1/1000, was used as the secondary. This image is courtesy of an Abreview submitted by Dr Hannah Whiteman
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Cytokeratin 8 antibody [M20](ab9023)
](\ps\datasheet\images\9\ab9023\Cytokeratin-8-Primary-antibodies-ab9023-1.jpg)
Ab9023 staining human normal liver parenchima tissue. Staining is localized to cell membrane.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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References for Cytokeratin 8 antibody [M20] (ab9023)
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This product has been used in: (two most recent references)
Bajpai M et al. Repeated exposure to acid and bile selectively induces colonic phenotype expression in a heterogeneous Barrett's epithelial cell line. Lab Invest 88:643-51 (2008). Flow Cyt; Human. PubMed: 18427553
Whiteman HJ et al. The role of S100P in the invasion of pancreatic cancer cells is mediated through cytoskeletal changes and regulation of cathepsin D. Cancer Res 67:8633-42 (2007). PubMed: 17875703
See all 4 publication references for this product.
If you publish research using ab9023 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Cytokeratin 8
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Cytokeratin 8 antibody [M20] - more information
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Cytokeratin 8 antibody [M20] - related products:
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Contact Abcam with a Technical Enquiry about Cytokeratin 8 antibody [M20] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - Cytokeratin 8 antibody [M20] (ab9023)](/index.html?datasheet=9023#ab9023_1.jpg)
![Immunocytochemistry/ Immunofluorescence - Cytokeratin 8 antibody [M20] (ab9023)](/index.html?datasheet=9023#ab9023_2.jpg)
![Immunocytochemistry/ Immunofluorescence - Cytokeratin 8 antibody [M20] (ab9023)](/index.html?datasheet=9023#ab9023_3.jpg)
