Recombinant Anti-Cytokeratin 8 antibody [SP102], prediluted (ab228149)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP102] to Cytokeratin 8, prediluted
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cytokeratin 8 antibody [SP102], prediluted
See all Cytokeratin 8 primary antibodies -
Description
Rabbit monoclonal [SP102] to Cytokeratin 8, prediluted -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human breast ductal carcinoma, breast, lung squamous cell carcinoma, placenta, thyroid, bladder, bladder transitional cell carcinoma, stomach, stomach adenocarcinoma, prostate, prostate adenocarcinoma and lung tissue.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: Tris buffered saline, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP102 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/1. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Ready to use. Primary antibody incubation for 30 minutes at room temperature. |
Notes |
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IHC-P
1/1. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Ready to use. Primary antibody incubation for 30 minutes at room temperature. |
Target
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Function
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
Tissue specificity
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. -
Involvement in disease
Cirrhosis -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsPhosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. -
Cellular localization
Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix. - Information by UniProt
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Database links
- Entrez Gene: 281269 Cow
- Entrez Gene: 3856 Human
- Entrez Gene: 16691 Mouse
- Entrez Gene: 100152077 Pig
- Entrez Gene: 25626 Rat
- Omim: 148060 Human
- SwissProt: P05786 Cow
- SwissProt: P05787 Human
see all -
Alternative names
- CARD2 antibody
- CK 8 antibody
- CK-8 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human lung tissue stained for Cytokeratin 8 using ab228149 in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Cytokeratin 8 using ab228149 in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human breast tissue stained for Cytokeratin 8 using ab228149 in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for Cytokeratin 8 using ab228149 in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human placenta tissue stained for Cytokeratin 8 using ab228149 in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human thyroid tissue stained for Cytokeratin 8 using ab228149 in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human bladder tissue stained for Cytokeratin 8 using ab228149 in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for Cytokeratin 8 using ab228149 in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human stomach tissue stained for Cytokeratin 8 using ab228149 in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Cytokeratin 8 using ab228149 in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human prostate tissue stained for Cytokeratin 8 using ab228149 in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for Cytokeratin 8 using ab228149 in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Cytokeratin 8 using ab228149 in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228149 has not yet been referenced specifically in any publications.