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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Overview
Product name
Anti-Cytokeratin 8 antibody
See all Cytokeratin 8 products (34) ...
Description
Chicken polyclonal to Cytokeratin 8
Tested applications
IHC-FoFr, ICC/IF, Flow Cyt, WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Recombinant fragment, corresponding to amino acids 391-483 of Human Cytokeratin 8.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgY
Research areas
Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Other
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14053 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
Flow Cyt
WB
IHC-Fr
Application notes
Flow Cyt: Use 1µg for 106 cells.
ICC/IF: Use at an assay dependent dilution (PMID 19651890).
IHC-P: 1/100.
IHC-Fr: 1/100.
IHC-FoFr: 1/250.
WB: 1/500. Predicted molecular weight: 54 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue specificity
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Involvement in disease
Defects in KRT8 are a cause of cirrhosis (CIRRH) [MIM:215600].
Sequence similarities
Belongs to the intermediate filament family.
Post-translational
modifications
Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
Cellular localization
Cytoplasm. Nucleus > nucleoplasm. Nucleus matrix.
Target information above from: UniProt accessionP05787
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- card2 antibody
- Cardiac autoantigen 2 120kD antibody
- CK 8 antibody
see all
Database links
- Entrez Gene: 3856 Human
- Entrez Gene: 16691 Mouse
- Entrez Gene: 25626 Rat
- Omim: 148060 Human
- SwissProt: P05787 Human
- SwissProt: P11679 Mouse
- SwissProt: Q10758 Rat
- Unigene: 533782 Human
see all
Anti-Cytokeratin 8 antibody images:
Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 8 antibody (ab14053)
ab14053 at 1/100 dilution detecting Cytokeratin 8 from mouse ovary tissue sections by immunohistochemistry (paraffin-embedded sections). The antibody was incubated with the tissue for 16 hours and then detected using Alexa-Fluor® 647 goat anti-chicken antibody.
This image is courtesy of an Abreview submitted by Albert Santamaria on 15 December 2005.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Cytokeratin 8 antibody (ab14053)
ab14053 staining adrenal gland tissue sections by IHC-FoFr. Sections were PFA fixed and blocked with 0.5% TNB for 30 minutes at 25°C. The primary antibody was diluted 1/250 and incubated with the sample for 18 hours at 4°C. An Alexa Fluor® 546 conjugated goat anti-chicken antibody, diluted 1/500, was used as the secondary. The image (taken at 80x) demonstrates glandular cells in the adrenal gland.
This image is courtesy of an anonymous Abreview
References for Anti-Cytokeratin 8 antibody (ab14053)
This product has been referenced in:
- Wu Xet al. Differentiation of the ductal epithelium and smooth muscle in the prostate gland are regulated by the Notch/PTEN-dependent mechanism. Dev Biol 356:337-49 (2011). IHC-P; Mouse.Read more (PubMed: 21624358) »
- Hay Tet al. Poly(ADP-ribose) polymerase-1 inhibitor treatment regresses autochthonous Brca2/p53-mutant mammary tumors in vivo and delays tumor relapse in combination with carboplatin. Cancer Res 69:3850-5 (2009). IHC-P; Mouse.Read more (PubMed: 19383921) »
See all 6 publications for this product
Publishing research using ab14053? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"