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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 8 antibody (ab59400)
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Western blot - Cytokeratin 8 antibody (ab59400)
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Immunocytochemistry/ Immunofluorescence-Cytokeratin 8 antibody(ab59400)
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Product Name
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Cytokeratin 8 antibody
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See all Cytokeratin 8 antibodies (30)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Cytokeratin 8
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Immunogen
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Synthetic non-phosphopeptide derived from human Cytokeratin 8 around the phosphorylation site of serine 431 (L-T-SP-P-G).
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/5000.
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human colon carcinoma tissue; EGF treated 293 cell extracts
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Other
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
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Relevance
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Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cytokeratin 8 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Cytokeratin 8 antibody images, references, products related to ab59400 and other tools.
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Cytokeratin 8 antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 8 antibody (ab59400)
Immunohistochemical analysis of paraffin embedded human colon carcinoma tissue using ab59400 at 1/50 dilution, in the presence (right) and absence (left) of immunising peptide. Then, a polymer secondary antibody was used for detection.
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Western blot - Cytokeratin 8 antibody (ab59400)
All lanes : Cytokeratin 8 antibody (ab59400) at 1/500 dilution
Lane 1 : EGF treated (200ng/ml, 30mins) 293 cell extracts
Lane 2 : EGF treated (200ng/ml, 30mins) 293 cell extracts with immunising peptide
Predicted band size : 54 kDa
Observed band size : 54 kDa
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Immunocytochemistry/ Immunofluorescence-Cytokeratin 8 antibody(ab59400)
ICC/IF image of ab59400 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59400, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to Cytokeratin 8
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Cytokeratin 8 antibody - more information
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Customer reviews (feedback) regarding Cytokeratin 8 antibody |
Customer FAQs regarding Cytokeratin 8 antibody |
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