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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Overview
Product name
Anti-Cytokeratin 8 antibody
See all Cytokeratin 8 products (34) ...
Description
Rabbit polyclonal to Cytokeratin 8
Tested applications
IHC-P, ICC/IF, WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic non-phosphopeptide derived from human Cytokeratin 8 around the phosphorylation site of serine 431 (L-T-SP-P-G).
Positive control
Human colon carcinoma tissue; EGF treated 293 cell extracts
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Other
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent concentration.
ICC/IF: Use a concentration of 1 µg/ml
WB: 1/500 - 1/1000.Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/5000ELISA: 1/5000
Target
Function
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue specificity
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Involvement in disease
Defects in KRT8 are a cause of cirrhosis (CIRRH) [MIM:215600].
Sequence similarities
Belongs to the intermediate filament family.
Post-translational
modifications
Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
Cellular localization
Cytoplasm. Nucleus > nucleoplasm. Nucleus matrix.
Target information above from: UniProt accessionP05787
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- card2 antibody
- Cardiac autoantigen 2 120kD antibody
- CK 8 antibody
see all
Database links
- Entrez Gene: 3856 Human
- Entrez Gene: 16691 Mouse
- Entrez Gene: 25626 Rat
- Omim: 148060 Human
- SwissProt: P05787 Human
- SwissProt: P11679 Mouse
- SwissProt: Q10758 Rat
- Unigene: 533782 Human
see all
Anti-Cytokeratin 8 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 8 antibody (ab59400)
Immunohistochemical analysis of paraffin embedded human colon carcinoma tissue using ab59400 at 1/50 dilution, in the presence (right) and absence (left) of immunising peptide. Then, a polymer secondary antibody was used for detection.
Western blot - Cytokeratin 8 antibody (ab59400)
All lanes : Anti-Cytokeratin 8 antibody (ab59400) at 1/500 dilution
Lane 1 : EGF treated (200ng/ml, 30mins) 293 cell extracts
Lane 2 : EGF treated (200ng/ml, 30mins) 293 cell extracts with immunising peptide
Predicted band size : 54 kDa
Observed band size : 54 kDa
Immunocytochemistry/ Immunofluorescence - Cytokeratin 8 antibody (ab59400)
ICC/IF image of ab59400 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59400, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 8 antibody (ab59400)
ab59400 staining Cytokeratin 8 in Human breast cancer cells xenografted into nude mice by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formalin and subjected to heat-mediated antigen retrieval in citrate buffer (0.1M Sodium Citrate) prior to blocking with 5% serum for 1 hour at 4°C. The primary antibody was diluted 1/200 in 5% goat serum in PBS and incubated with the sample for 12 hours at 4°C. A Biotin-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/1000.
This image is courtesy of an Abreview provided by Megha Rajaram.
References for Anti-Cytokeratin 8 antibody (ab59400)
This product has been referenced in:
- Scully Set al. Inhibitory activity of YKL-40 in mammary epithelial cell differentiation and polarization induced by lactogenic hormones: a role in mammary tissue involution. PLoS One 6:e25819 (2011). IHC-P; Mouse.Read more (PubMed: 21991364) »
- Driskell Iet al. The histone methyltransferase Setd8 acts in concert with c-Myc and is required to maintain skin. EMBO J : (2011).Read more (PubMed: 22117221) »
See all 3 publications for this product
Publishing research using ab59400? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"