Recombinant Anti-Cytokeratin 9 antibody [EPR10932] (ab167398)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10932] to Cytokeratin 9
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cytokeratin 9 antibody [EPR10932]
See all Cytokeratin 9 primary antibodies -
Description
Rabbit monoclonal [EPR10932] to Cytokeratin 9 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human Cytokeratin 9.
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Positive control
- WB: NCI-H460, A431, HeLa, and HL-60 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10932 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab167398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 62 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 62 kDa. |
Target
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Function
May serve an important special function either in the mature palmar and plantar skin tissue or in the morphogenetic program of the formation of these tissues. Plays a role in keratin filament assembly. -
Tissue specificity
Expressed in the terminally differentiated epidermis of palms and soles. -
Involvement in disease
Defects in KRT9 are a cause of palmoplantar keratoderma epidermolytic (EPPK) [MIM:144200]; also abbreviated as EHPPK. EPPK is a dermatological disorder characterized by diffuse thickening of the epidermis on the entire surface of palms and soles sharply bordered with erythematous margins. Some patients may present with knuckle pads, thick pads of skin appearing over the proximal phalangeal joints. -
Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
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Database links
- Entrez Gene: 3857 Human
- Entrez Gene: 107656 Mouse
- Entrez Gene: 266717 Rat
- Omim: 607606 Human
- SwissProt: P35527 Human
- SwissProt: Q6RHW0 Mouse
- SwissProt: Q8CIS9 Rat
- Unigene: 654569 Human
see all -
Alternative names
- CK 9 antibody
- CK-9 antibody
- Cytokeratin 9 antibody
see all
Images
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All lanes : Anti-Cytokeratin 9 antibody [EPR10932] (ab167398) at 1/10000 dilution
Lane 1 : NCI-H460 (Human Large Cell Lung Cancer epithelial cell) whole cell lysates
Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 4 : HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDaBlocking buffer and concentration 5% NFDM/TBST
Diluting buffer and concentration 5% NFDM/TBSTExposure time:
Lane 1 to 3: 30 seconds
Lane 4: 180 seconds
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab167398 has been referenced in 2 publications.
- Gong P et al. Tissue Plasminogen Activator Causes Brain Microvascular Endothelial Cell Injury After Oxygen Glucose Deprivation by Inhibiting Sonic Hedgehog Signaling. Neurochem Res N/A:N/A (2018). PubMed: 30552546
- Rice RH et al. Distinguishing ichthyoses by protein profiling. PLoS One 8:e75355 (2013). WB ; Human . PubMed: 24130705