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Cytomegalovirus Glycoprotein B protein (ab43040)

Submit an Abreview Q&A (5)

Overview

  • Product nameCytomegalovirus Glycoprotein B protein
  • Protein descriptionThe protein contains the CMV Glycoprotein B immunodominant regions with a ~26kDa tag. (E.coli derived recombinant protein).
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesSepharose derived purification.
  • Biological activityImmunoreactive with sera of CMV infected individuals.
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, 25mM Tris HCl, 1mM EDTA, pH 7.2
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab43040 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    ELISA ELISA: Use at an assay dependent dilution.
    WB WB: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        CMVCMV glycoprotein BgB
        Glycoprotein BGlycoprotein GP55HCMVHHV5 glycoprotein BUL55
      see all
  • RelevanceHuman cytomegalovirus (HCMV) glycoprotein B (gB) is an abundant virion envelope protein that has been shown to be essential for the infectivity of HCMV. HCMV gB is also one of the most immunogenic virus-encoded proteins, and a significant fraction of virus neutralizing antibodies are directed at gB.
  • Cellular localizationVirus envelope.

    • References for Cytomegalovirus Glycoprotein B protein (ab43040)

    ab43040 has not yet been referenced specifically in any publications.

    Publishing research using ab43040 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab43040 Submit a Question for ab43040

    Displaying 1 - 5 of 5 results for Abreviews and Q&A

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    Votes: Highest First

    Hello,

    I am following up from a phone call that I had last week. I requested information on the protein sequence of the following peptides:

    Cytomegalovirus pp65 IE protein (ab54103)

    Cytomegalovirus Glycoprotein B protein (...

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    Thank you for your inquiry.

    I can confirm that the immunogen for ab43040 is from within the amino acid range 11-67. I am sorry the exact sequence is proprietary.

    Unfortunately, we are not able to release the immunogen sequence forab5...

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    I was wondering if it was normal that I detect two bands when I perform a Western Blot against the gB protein with a CMV anti-gB monoclonal antibody (2F12 clone). It looks like a doublet on the gel, around 37 kDa of MW. -4ug loaded -CMV gB mouse monoclona...

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    Thank you for supplying that information.


    I have been in contact with the lab and they suggested that maybe the PAGE sample was heated too long (5 minutes) prior to loading. It is most likely that a specific smallmolecular weight amino acid...

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    Hello, I was wondering if it was normal that I detect two bands when I perform a Western Blot against the gB protein with a CMV anti-gB monoclonal antibody (2F12 clone). It looks like a doublet on the gel, around 37 kDa of MW. -4ug loaded -CMV gB mouse mo...

    Read More

    Thank you for contacting Abcam.


    I have been in contact with the lab about your enquiry as to whether a doublet was expected on a western blot, when you ran ab43040 on a western blot. They have asked for some more information:

    Read More

    What are the amino acids of Cytomegalovirus Glycoprotein B fused to GST fusion in this protein?

    Sorry for the late reply. The protein contains the CMV gB immunodominant regions without the signal peptide. I hope this is helpful. Please contact us again if you have any further questions.

    What is the detected molecular weight of this product? When I ran a SDS-PAGE, I only get a strong band at around 30kDa but your datasheet states that the predicted size is 102kDa.

    Thank you for your enquiry. This product was made from a 57 amino acid long sequence and a GST fusion. The total molecular weight should be 32.2kDA. Therefore, please rest assured that the band you are seeing is indeed the Cytomegalovirus Glycoprotein...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"