Anti-Cytomegalovirus pp65 antibody [3A12] (ab6503)

Overview

  • Product name
    Anti-Cytomegalovirus pp65 antibody [3A12]
    See all Cytomegalovirus pp65 primary antibodies
  • Description
    Mouse monoclonal [3A12] to Cytomegalovirus pp65
  • Specificity
    Cytomegalovirus pp65 (Lower Matrix Protein).
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Immunogen

    Cytomegalovirus

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Phosphate buffered saline
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    3A12
  • Myeloma
    NS1/1-Ag4-1
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/16000.
ICC/IF 1/3200.

Target

  • Relevance
    Cytomegalovirus is a member of the herpes virus group, which includes herpes simplex virus types 1 and 2, varicella zoster virus (which causes chicken pox), and Epstein Barr virus (which causes infectious mononucleosis). These viruses share a characteristic ability to remain dormant within the body over a long period. CMV viral genes are co-ordinately expressed in groups at various times after infection. Early viral proteins are expressed in the nucleus of infected cells within 3 to 24 hours of infection prior to the commencement of viral DNA replication. This is followed by expression of the early intermediate genes, which encode enzymes required for viral DNA replication. After 48 to 72 hours, a number of late viral antigens may be demonstrated in the nuclei and cytoplasm of infected cells. pp65 is a 65kD phosphorylated glycoprotein and is the most abundant of the late antigens.
  • Alternative names
    • 65 kDa matrix phosphoprotein antibody
    • 65 kDa phosphoprotein antibody
    • Tegument protein pp65 antibody
    • Tegument protein UL83 antibody

References

This product has been referenced in:
  • Huang Y  et al. Interaction between HCMV pUL83 and human AIM2 disrupts the activation of the AIM2 inflammasome. Virol J 14:34 (2017). Read more (PubMed: 28219398) »
  • Zimmermann A  et al. Checks and balances between human cytomegalovirus replication and indoleamine-2,3-dioxygenase. J Gen Virol 95:659-70 (2014). WB . Read more (PubMed: 24337170) »

See all 5 Publications for this product

Customer reviews and Q&As

Thank you for your inquiry. As you may have seen already, we have a number of HCMV pp65 antibodies available in our catalog (such as ab6503, ab8250, ab31624, ab49214, ab53489). These were raised against the pp65 protein, however, unfortunately the ...

Read More

I put your question forward to the originator but they said that the epitope has not been characterised so they do not have the information. I am sorry we cannot be of help to you.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up