D-Lactate Assay Kit (Colorimetric) (ab83429)

Overview

  • Product nameD-Lactate Assay Kit (Colorimetric)See all D-Lactate kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 0.01 mM
  • Range
    0.01 mM - 10 mM
  • Assay time
    0h 40m
  • Product overview

    Abcam's D-Lactate Assay Kit (Colorimetric)Kit provides a fast, easy way to accurately measure D-lactate in a variety of biological samples. In this kit, D-lactate is specifically oxidized by D-lactate dehydrogenase and generates proportional color (λmax = 450 nm). The kit detects D-Lactate in samples such as serum, plasma, cells, culture and fermentation media.
    Visit our FAQs page for tips and troubleshooting.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceD-Lactate production in mammals, mainly due to the glyoxalase pathway, is extremely low, with normal serum concentrations in the nano to micromolar range. Typically, elevated D-lactate levels which can rise to millimolar levels, are due to bacterial infection or short bowel syndrome in humans. Abnormally high concentrations of D-lactate are considered indicative of sepsis, ischemia or trauma. Due to slow metabolism and excretion, high D-lactate can cause acidosis and encephalopathy.
  • Alternative names
    • D-Lactic acid

Applications

Our Abpromise guarantee covers the use of ab83429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Functional Studies Use at an assay dependent dilution.

D-Lactate Assay Kit (Colorimetric) images

  • D-Lactate Standard Curve. The assay is performed according to the kit instruction. The assay specifically detects D-Lactate in the presence of up to 1000X L-Lactate.

Protocols

References for D-Lactate Assay Kit (Colorimetric) (ab83429)

ab83429 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. Yes, D-lactate is produced in very low levels in mammals unless there are certain bacterial infections or trauma. L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism and is present in b...

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Urine samples would also have NADH. Therefore, the background control step should be run.
Since NADH is one of the reaction intermediates in this assay, we want to make certain that any readings we see are due to the assay reaction of D-Lactate co...

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Besides completely depressing the plunger on the pipette tip, the lab suggested also the following:

In addition, the customer can try to point the pipette tip towards the side walls of the wells instead of the bottom. That way the bubbles...

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Thank you for taking the time to complete our questionnaire and providing this further information. I am sorry to hear you have had difficulty obtaining satisfactory results from our D-Lactate Assay Kit (Colorimetric) (ab83429) and our Pyruvate Assay K...

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Thank you for your enquiry regarding ab83429.

As long as the band width of detection on the instrument says ± 50, you can use 500 nm instead of 450 nm. Otherwise it will compromise the efficiency of the assay.

I hope this hel...

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Please let the Dr B. know the following:

You can directly use different doses of serum/plasma with this assay. Just make up the volume in the wells to 50 µl with the assay buffer.

All readings have to be made at 450 nm. Pleas...

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Thank you for contacting us.

I am not familiar with the contents of MRSB media, but in general cell culture supernatants will be compatible with these kits. If the media contains serum, we recommend deproteinizing the samples using our 10 kDa...

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Thank you for your inquiry.

1.) I can confirm that urine should be centrifuged for 30 minutes at 1500g. Then the samples should be stored at -80C and / orde- proteinised with ab93349 (10kD spin column).

http://www.abcam.com/index.ht...

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Thank you for your inquiry.

I am not personally familiar with the Roche Cobas C501, however this is a colorimetric assay, so as long as your plate reader measures at 450 nM, it should be fine.

The protocol with all the volume infoi...

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Thank you for your inquiry.  I heard back from the supplying lab that the exact reaction products and mechanisms of the kit are considered to be proprietary information. The enzymes convert D-Lactate to a product (proprietary) which then reacts with th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"