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D-Lactate Assay Kit (Colorimetric) (ab83429)

Q&A (13)

Overview

  • Product nameD-Lactate Assay Kit (Colorimetric)
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 0.01 mM
  • Range
    0.01 mM - 10 mM
  • Assay time
    0h 40m
  • Product overview

    Abcam's D-Lactate Assay Kit (Colorimetric)Kit provides a fast, easy way to accurately measure D-lactate in a variety of biological samples. In this kit, D-lactate is specifically oxidized by D-lactate dehydrogenase and generates proportional color (λmax = 450 nm). The kit detects D-Lactate in samples such as serum, plasma, cells, culture and fermentation media.
    Visit our FAQs page for tips and troubleshooting.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceD-Lactate production in mammals, mainly due to the glyoxalase pathway, is extremely low, with normal serum concentrations in the nano to micromolar range. Typically, elevated D-lactate levels which can rise to millimolar levels, are due to bacterial infection or short bowel syndrome in humans. Abnormally high concentrations of D-lactate are considered indicative of sepsis, ischemia or trauma. Due to slow metabolism and excretion, high D-lactate can cause acidosis and encephalopathy.
    • Alternative names
        D-Lactic acid

    Applications

    Our Abpromise guarantee covers the use of ab83429 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    D-Lactate Assay Kit (Colorimetric) images

    • Functional Studies - D-Lactate Colorimetric Assay Kit (ab83429)
      Functional Studies - D-Lactate Colorimetric Assay Kit (ab83429)
      D-Lactate Standard Curve. The assay is performed according to the kit instruction. The assay specifically detects D-Lactate in the presence of up to 1000X L-Lactate.

    Protocols

    References for D-Lactate Assay Kit (Colorimetric) (ab83429)

    ab83429 has not yet been referenced specifically in any publications.

    Publishing research using ab83429 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

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    Displaying 1 - 10 of 13 results for Abreviews and Q&A

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    Hi,

    I was looking for D-lactate assay kit and came to L-Lactate with Abcam. Would you please kindly advice me on the difference between D & L-Lactate and their measurement in clinical application. I know that D is at lower concentration vs ...

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    Thank you for contacting us. Yes, D-lactate is produced in very low levels in mammals unless there are certain bacterial infections or trauma. L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism and is present in bloo...

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    Customer has kindly contacted us to inquire about use of the NADH background correction step when using this kit. They are using mouse urine samples and are unsure if this step will be needed.

    Urine samples would also have NADH. Therefore, the background control step should be run.
    Since NADH is one of the reaction intermediates in this assay, we want to make certain that any readings we see are due to the assay reaction of D-Lactate conve...

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    D-Lactate colorimetric assay, product # ab83429.
    When using your assay for the first time, I noticed that when pipetting accurate volumes of the reaction mixture (namely the assay buffer), there was a significant quantity of bubbles produced due to th...

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    Besides completely depressing the plunger on the pipette tip, the lab suggested also the following:

    In addition, the customer can try to point the pipette tip towards the side walls of the wells instead of the bottom. That way the bubbles wo...

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    A. General Information
    - Catalogue number: ab83429; ab65342
    - Lot number:
    - Purchase order number/ order date or preferably the Abcam order number:
    - The kits were stored at -20C.
    B. Description of the problem
    - Are the signal...

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    Thank you for taking the time to complete our questionnaire and providing this further information. I am sorry to hear you have had difficulty obtaining satisfactory results from our D-Lactate Assay Kit (Colorimetric) (ab83429) and our Pyruvate Assay Kit ...

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    Can they read at 500nm in place of 450nm ?

    Regards

    Thank you for your enquiry regarding ab83429.

    As long as the band width of detection on the instrument says ± 50, you can use 500 nm instead of 450 nm. Otherwise it will compromise the efficiency of the assay.

    I hope this helps ...

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    Hell,



    Please check and reply.



    Customer want to check D-lactate from blood through our kit ab 83429.



    I have sent her the protocol and datasheet . She has the following quiries .

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    Please let the Dr B. know the following:

    You can directly use different doses of serum/plasma with this assay. Just make up the volume in the wells to 50 µl with the assay buffer.

    All readings have to be made at 450 nm. Please d...

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    I am working in a research project and I need to measure the levels of D-lactate and L-lactate in MRSB culture supernatants, are the kits sale by your company compatible with this type of media . Is there any interference due to the media color?

    Thank you for contacting us.

    I am not familiar with the contents of MRSB media, but in general cell culture supernatants will be compatible with these kits. If the media contains serum, we recommend deproteinizing the samples using our 10 kDa cu...

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    Dear Sir,
    I'm interesting to use your product: D-Lactate ab83429 (Abcam) using
    urine sample
    I want to ask you according to preparation urine sample.
    1. Can we centrifuge the urine sample in 1500 g 4oC in 15 min? is it Ok?

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    Thank you for your inquiry.

    1.) I can confirm that urine should be centrifuged for 30 minutes at 1500g. Then the samples should be stored at -80C and / orde- proteinised with ab93349 (10kD spin column).

    http://www.abcam.com/index.html?...

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    Inquiry: Can the d-lactate kit be used on an automated biochemistry analzyer such as the Roche Cobas c501? if so, do you have an application or suggestions as to how the application can be modified?

    Thank you for your inquiry.

    I am not personally familiar with the Roche Cobas C501, however this is a colorimetric assay, so as long as your plate reader measures at 450 nM, it should be fine.

    The protocol with all the volume infois l...

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    What end product from the reaction is being measured in this kit?  Other kits that are commercially available measure the end product of NADH, but it's measured at 340nm and your kit measures at 450nm instead.  What is being measured at 450nm?

    Thank you for your inquiry.  I heard back from the supplying lab that the exact reaction products and mechanisms of the kit are considered to be proprietary information. The enzymes convert D-Lactate to a product (proprietary) which then reacts with the p...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"