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Anti-DAB2 antibody [EP2297Y] (ab76253)

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Overview

Product name

Anti-DAB2 antibody [EP2297Y]
See all DAB2 products (5) ...

Description

Rabbit monoclonal [EP2297Y] to DAB2

Tested applications

WB, IP, ICC, Flow Cytmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues near C terminal of human DAB2

Positive control

Human pancreas tissue lysates.

General notes

Produced under U.S. Patent No. 5,675,063

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP2297Y

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases

  • Western blot - DAB2 antibody [EP2297Y] (ab76253)Western blot - DAB2 antibody [EP2297Y] (ab76253) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab76253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/2000 - 1/10,000.De...Read more →

    WB: 1/2000 - 1/10,000.Detects a band of approximately 105 kDa (predicted molecular weight: 83 kDa).

  • IP

     IP: 1/20

    IP: 1/20

  • ICC

     ICC: 1/100 - 1/250.

    ICC: 1/100 - 1/250.

  • Flow Cyt

     Flow Cyt: 1/50

    Flow Cyt: 1/50

Application notes

Is unsuitable for or IHC-P.

Target

Relevance

DAB2 is a component of the CSF1 signal transduction pathway. DAB2 mRNA is expressed in normal ovarian epithelial cells but is down regulated or absent from ovarian carcinoma cell lines. The down regulation of DAB2 may play an important role in ovarian carcinogenesis. This gene was initially named DOC2 (for Differentially expressed in Ovarian Cancer) and is distinct from the DOC2A and DOC2B genes (for double C2 like domains, alpha and beta).

Cellular localization

Cytoplasmic vesicle; clathrin coated vesicle; clathrin coated vesicle membrane. Cell membrane; coated pit; clathrin coated pit. Note: Colocalizes with large insert containing isoforms of MYO6 at clathrin coated pits/vesicles.

Database links

Anti-DAB2 antibody [EP2297Y] images:

  Western blot - DAB2 antibody [EP2297Y] (ab76253)

Western blot - DAB2 antibody [EP2297Y] (ab76253)

Anti-DAB2 antibody [EP2297Y] (ab76253) at 1/2000 dilution + human pancreas tissue lysates at 10 µg

Secondary
goat anti-rabbit HRP at 1/2000 dilution

Predicted band size : 83 kDa
Observed band size : 105 kDa (why is the actual band size different from the predicted?)

References for Anti-DAB2 antibody [EP2297Y] (ab76253)

ab76253 has not yet been referenced specifically in any publications.

Publishing research using ab76253? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"