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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Overview
Product name
Anti-DAB2 antibody
See all DAB2 products (5) ...
Description
Mouse polyclonal to DAB2
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length protein, corresponding to amino acids 1-770 of Human DAB2 (AAH03064.1).
Positive control
Human liver tissue, HeLa cell, transfected 293T cell lysates and HeLa cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab88590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 82 kDa.
ICC/IF: Use a concentration of 10 µg/ml
Target
Relevance
DAB2 is a component of the CSF1 signal transduction pathway. DAB2 mRNA is expressed in normal ovarian epithelial cells but is down regulated or absent from ovarian carcinoma cell lines. The down regulation of DAB2 may play an important role in ovarian carcinogenesis. This gene was initially named DOC2 (for Differentially expressed in Ovarian Cancer) and is distinct from the DOC2A and DOC2B genes (for double C2 like domains, alpha and beta).
Cellular localization
Cytoplasmic vesicle; clathrin coated vesicle; clathrin coated vesicle membrane. Cell membrane; coated pit; clathrin coated pit. Note: Colocalizes with large insert containing isoforms of MYO6 at clathrin coated pits/vesicles.
Alternative names
- DAB 2 antibody
- Differentially expressed protein 2 antibody
- Disabled (Drosophila) homolog 2 (mitogen responsive phosphoprotein) antibody
see all
Database links
- Entrez Gene: 1601 Human
- Omim: 601236 Human
- SwissProt: P98082 Human
- Unigene: 481980 Human
Anti-DAB2 antibody images:
Western blot - DAB2 antibody (ab88590)
Anti-DAB2 antibody (ab88590) at 1 µg/ml + human liver tissue lysate at 50 µg
developed using the ECL technique
Predicted band size : 82 kDa
Observed band size : 82 kDa
Western blot - DAB2 antibody (ab88590)
Anti-DAB2 antibody (ab88590) at 1 µg/ml + HeLa cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 82 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
Western blot - DAB2 antibody (ab88590)
All lanes : Anti-DAB2 antibody (ab88590) at 1 µg/ml
Lane 1 : cell lysate from DAB2 transfected 293T cells
Lane 2 : cell lysate from non transfected 293T cells
Lysates/proteins at 25 µg per lane.
developed using the ECL technique
Predicted band size : 82 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - DAB2 antibody (ab88590)
ab88590 at 10 µg/ml detecting DAB2 in HeLa cells by immunofluorescence.
References for Anti-DAB2 antibody (ab88590)
ab88590 has not yet been referenced specifically in any publications.
Publishing research using ab88590? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"