D4 GDI antibody (ab12427)

Product Name 

D4 GDI antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to D4 GDI

Immunogen 

Synthetic peptide: KAPEPHVEEDDDDELDSKC conjugated to KLH, corresponding to amino acids 4-21 of Human D4 GDI.
BLAST 'KAPEPHVEEDDDDELDSKC' with or

Reacts with 
(species key)

Hu

Specificity 

Ab12427 specifically reacts with D4 GDI and does not recognize Rho-GDI or GDI-3.

Tested applications 
(see key)

IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IP: 4 µg is recommended to immunoprecipitate D4 GDI from 500 µg of Jurkat RIPA lysate.
WB: Use at a concentration of 0.5 - 1 µg/ml. This concentration is recommended for blotting using RIPA lysates from human A431, HL-60 and Jurkat cells, anti-rabbit IgG-peroxidase conjugate and a chemiluminescent detection system. Predicted molecular weight: 23 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

RIPA lysates from human A431, HL-60 and Jurkat cells,

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly

Relevance 

D4 GDI belongs to the family of Rho GDIs or GDP dissociation inhibitors for the Rho/Rac family of small G proteins; consisting of RhoGDI 1, D4 GDI/RhoGDI2 and Rho GDI3. It inhibits the release of GDP from the Rho proteins, essential for GTP loading and activation of GTPase activity. D4 GDI is located in the cytoplasm and in involved in the regulation of the membrane association and dissociation cycle of Rho/Rac proteins. It is known to be a substrate for caspase 3 during Fas mediated apoptosis.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: 0.035% Sodium Azide
Constituents: 30% Glycerol, 0.105M Sodium Chloride, 0.07M Tris-glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The IgG fraction of antiserum is purified using protein A.

Form 

Liquid

Concentration 

0.700 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Customer reviews (feedback) regarding D4 GDI antibody

Customer FAQs regarding D4 GDI antibody

Protocols for D4 GDI antibody

Price and availability of products related to D4 GDI antibody

D4 GDI antibody - related products: