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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
Overview
Product name
Anti-DAAM1 antibody - Aminoterminal end
See all DAAM1 products (4) ...
Description
Rabbit polyclonal to DAAM1 - Aminoterminal end
Tested applications
ELISA, IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide from the N terminal region of human DAAM1.
Positive control
WB: 293 cell lysate IHC-P: human brain tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab71327 is purified through a protein A column, followed by peptide affinity purification.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
IHC-P: 1/10 - 1/50.
WB: 1/50 - 1/100.Detects a band of approximately 130 kDa (predicted molecular weight: 123 kDa).
Target
Relevance
DAAM1 (Dishevelled associated activator of morphogenesis 1) is a diaphanous-related formin which binds to dishevelled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex assembly. It is an essential component of noncanonical Wnt signaling. DAAM1 also communicates with Rho GTPases, CIP4 and Src in the regulation of the signalling pathways that co-ordinate the dynamics of the actin filament system. DAAM1 may be a coordinator of endocytosis and cytoskeletal remodeling.
Cellular localization
Cytoplasmic. Note: Perinuclear.
Alternative names
- DAAM 1 antibody
- Dishevelled associated activator of morphogenesis 1 antibody
- FLJ41657 antibody
- KIAA0666 antibody
Database links
- Entrez Gene: 23002 Human
- Omim: 606626 Human
- SwissProt: Q9Y4D1 Human
- Unigene: 654934 Human
Anti-DAAM1 antibody - Aminoterminal end images:
Western blot - DAAM1 antibody - Aminoterminal end (ab71327)
All lanes : Anti-DAAM1 antibody - Aminoterminal end (ab71327) at 1/50 dilution
Lane 1 : 293 cell lysate, nontransfected
Lane 2 : 293 cell lysate, transfected with the DAAM1 gene
Lysates/proteins at 2 µg per lane.
Predicted band size : 123 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DAAM1 antibody - Aminoterminal end (ab71327)
ab71327, at a 1/10 dilution, staining DAAM1 in formalin fixed and paraffin embedded human brain tissue by Immunohistochemistry. A peroxidase conjugated secondary antibody was then used, followed by DAB staining.
References for Anti-DAAM1 antibody - Aminoterminal end (ab71327)
ab71327 has not yet been referenced specifically in any publications.
Publishing research using ab71327? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"