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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
Overview
Product name
Anti-DAAM1 antibody - C-terminal
See all DAAM1 products (4) ...
Description
Rabbit polyclonal to DAAM1 - C-terminal
Tested applications
WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide from the N-terminal region of human DAAM1, conjugated to KLH.
Positive control
Paraffin embedded human brain tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody was purified through a protein A column, followed by immunogen affinity purification.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/50 - 1/100.Detects a band of approximately 123 kDa (predicted molecular weight: 123 kDa).
IHC-P: 1/10 - 1/50.
ELISA
ELISA: 1/1000ELISA: 1/1000
Target
Relevance
DAAM1 (Dishevelled associated activator of morphogenesis 1) is a diaphanous-related formin which binds to dishevelled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex assembly. It is an essential component of noncanonical Wnt signaling. DAAM1 also communicates with Rho GTPases, CIP4 and Src in the regulation of the signalling pathways that co-ordinate the dynamics of the actin filament system. DAAM1 may be a coordinator of endocytosis and cytoskeletal remodeling.
Cellular localization
Cytoplasmic. Note: Perinuclear.
Alternative names
- DAAM 1 antibody
- Dishevelled associated activator of morphogenesis 1 antibody
- FLJ41657 antibody
- KIAA0666 antibody
Database links
- Entrez Gene: 23002 Human
- Omim: 606626 Human
- SwissProt: Q9Y4D1 Human
- Unigene: 654934 Human
Anti-DAAM1 antibody - C-terminal images:
Western blot - DAAM1 antibody (ab71499)
All lanes : Anti-DAAM1 antibody - C-terminal (ab71499) at 1/100 dilution
Lane 1 : Non-transfected 293 cell lysate
Lane 2 : DAAM1 transfected 293 cell lysate
Lysates/proteins at 2 µg per lane.
Predicted band size : 123 kDa
Observed band size : 123 kDa
Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DAAM1 antibody (ab71499)
Immunohistochemical analysis of paraffin embedded human brain tissue using ab71499 at 1/10 dilution.
References for Anti-DAAM1 antibody - C-terminal (ab71499)
ab71499 has not yet been referenced specifically in any publications.
Publishing research using ab71499? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"