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All lanes : Anti-DAAM2 antibody (ab72764) at 0.5 µg/ml
Lane 1 : human skeletal muscle lysate
Lane 2 : human skeletal muscle lysate with immunizing peptide
Lane 3 : mouse skeletal muscle lysate
Predicted band size : 124 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
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