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Anti-DAAM2 antibody (ab72764)

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If your product does not perform as described on this datasheet, we will refund or replace your product...

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This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab72764 for help.

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1 question for ab72764

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Question 1

Tuesday 21-February-2012

We order recently the following reference from your company:
Anti-DAAM2 antibody (ab72764).
We tested the Ab using our lab standard conditions on mouse muscle lysate.
We obtain much more band than on the picture showed in your website.
Could you tell me if specific conditions (for tissue extraction / blocking...) are necessary ?

ANSWER:

 

Thank you for contacting us and sorry for the delay of our reply. I am sorry to hear that this antibody is not providing satisfactory results.

Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab72764:

For the blocking step, 5% milk was used.
The primary antibody was incubated overnight at 4ºC, at a concentration of 0.5 - 1 µg/ml, diluted in 1% milk / TBST.



Should the suggestions not improve the results, please do let me know.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

Since at Abcam we are concern about the quality of the products we supply, I would like to ask you further information regarding the samples you tested and the protocol you used. Could you please fill in the attached questionnaire? We will use those data to investigate the source of the problem with this product.

I hope this information is helpful, and I thank you for your cooperation.

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